Ok Evren, Adanir Necdet, Hakki Sema
Department of Endodontics, Faculty of Dentistry, Sifa University, Izmir, Turkiye.
Department of Endodontics, Faculty of Dentistry, Suleyman Demirel University, Isparta, Turkiye.
Eur J Dent. 2015 Jan-Mar;9(1):6-10. doi: 10.4103/1305-7456.149630.
The purpose of this in vitro study was to compare the cytotoxicity of 0.5-4.5 origanum extract solution (OES), 2% chlorhexidine gluconate (CHX) and 5.25% sodium hypochlorite (NaOCl) with WST-1 test on human periodontal ligament (hPDL) fibroblasts.
About 0.5-4.5% OES, 2% CHX and 5.25% NaOCl solutions cytotoxicity was evaluated with cell culture test using PDL fibroblasts. Viability of hPDL cells was evaluated with WST-1 (Cell Proliferation Reagent WST-1 Roche) test at 1, 24 and 72(nd) h. hPDL cells were plated at 20 × 10(3) cells per well in 96-well plates. Absorbance values were read in optical density 480 nm by ELISA plate reader spectrophotometer. The statistical differences between various groups were evaluated using one-way ANOVA, post-hoc Duncan's Multiple Range test using SAS software. Statistically, a significant difference was considered at P < 0.001.
According to the 1-h cytotoxicity results, 0.5% OES showed the least cytotoxic effect in test groups. There were not found any statistical significance between 1% OES and 2% CHX. About 5.25% NaOCl showed more cytotoxic effect than 1% OES and 2% CHX. In 24 and 72 h, different concentrations of OES, 5.25% NaOCl, 2% CHX solutions showed similar cytotoxic effect.
Based on these results, 1% OES and 2% CHX showed similar results and less cytotoxic effect than 5.25% NaOCl. It could be considered as a favorable solution concentration when OES was used as root canal irrigation solution.
本体外研究的目的是通过WST-1试验比较0.5 - 4.5%牛至提取物溶液（OES）、2%葡萄糖酸氯己定（CHX）和5.25%次氯酸钠（NaOCl）对人牙周膜（hPDL）成纤维细胞的细胞毒性。
使用牙周膜成纤维细胞通过细胞培养试验评估约0.5 - 4.5% OES、2% CHX和5.25% NaOCl溶液的细胞毒性。在第1、24和72小时用WST-1（细胞增殖试剂WST-1，罗氏公司）试验评估hPDL细胞的活力。将hPDL细胞以每孔20×10³个细胞接种于96孔板中。通过酶联免疫吸附测定（ELISA）酶标仪分光光度计在480 nm光密度下读取吸光度值。使用SAS软件通过单因素方差分析、事后邓肯多重极差检验评估各组之间的统计学差异。统计学上，P < 0.001被认为有显著差异。
根据1小时细胞毒性结果，0.5% OES在试验组中显示出最小的细胞毒性作用。1% OES和2% CHX之间未发现任何统计学差异。约5.25% NaOCl显示出比1% OES和2% CHX更强的细胞毒性作用。在24和72小时，不同浓度的OES、5.25% NaOCl、2% CHX溶液显示出相似的细胞毒性作用。
基于这些结果，1% OES和2% CHX显示出相似的结果，且细胞毒性作用比5.25% NaOCl小。当OES用作根管冲洗液时，它可被视为一种合适的溶液浓度。
