Cho Yong Wun, Han Yong Seop, Chung In Young, Kim Seong Jae, Seo Seong Wook, Yoo Ji Myong, Park Jong Moon
Department of Ophthalmology, Gyeongsang National University School of Medicine, Jinju 660-702, Korea.
Department of Ophthalmology, Gyeongsang National University School of Medicine, Jinju 660-702, Korea ; Gyeongsang Institute of Health Science, Gyeongsang National University, Jinju 660-702, Korea.
Int J Ophthalmol. 2014 Dec 18;7(6):952-8. doi: 10.3980/j.issn.2222-3959.2014.06.07. eCollection 2014.
To examine the effect of intravitreal adenoviral vector-mediated tristetraprolin (Ad-TTP) on VEGF mRNA expression in a rat model of laser-induced choroidal neovascularization.
Ad-TTP was prepared using a commercial kit. Retinal laser-induced photocoagulation (10 spots per eye) was performed on rats in this experimental choroidal neovascularization (CNV) model. Rats were divided into four groups: control (single intravitreal injection of balanced salt solution, n=10), laser-induced CNV (photocoagulation only, n=20), laser-induced CNV plus Ad-TTP injection (photocoagulation plus a single intravitreal Ad-TTP injection, n=20) and Ad-TTP injection only (n=10). Changes in choroidal morphology were evaluated in ten rats in the laser only and the laser plus Ad-TTP groups. Two weeks after laser injury, the size of CNV was calculated by perfusion with high-molecular-weight fluorescein isothiocyanate (FITC)-dextran. VEGF mRNA expression in retina-choroid tissue from ten rats in each group was measured by reverse transcription polymerase chain reaction (RT-PCR).
Two weeks after treatment, the area of laser-induced CNV was reduced by approximately 60% in the rats given the Ad-TTP injection compared with that in the laser-only group. There was a tendency toward decreased VEGF mRNA expression in the Ad-TTP injection groups.
A single intravitreal injection of Ad-TTP significantly suppressed CNV size in this experimental laser-induced CNV model. Ad-TTP injection also decreased VEGF mRNA expression compared with that in the laser-induced CNV group. The present study is meaningful as the first study to investigate the effect of tristetraprolin delivered via intravitreal injection.
在激光诱导的脉络膜新生血管大鼠模型中，研究玻璃体内腺病毒载体介导的锌指蛋白36（Ad-TTP）对血管内皮生长因子（VEGF）mRNA表达的影响。
使用商业试剂盒制备Ad-TTP。在此实验性脉络膜新生血管（CNV）模型中，对大鼠进行视网膜激光诱导光凝（每只眼10个光斑）。大鼠分为四组：对照组（玻璃体内单次注射平衡盐溶液，n = 10）、激光诱导CNV组（仅光凝，n = 20）、激光诱导CNV加Ad-TTP注射组（光凝加单次玻璃体内Ad-TTP注射，n = 20）和仅Ad-TTP注射组（n = 10）。在仅激光组和激光加Ad-TTP组的10只大鼠中评估脉络膜形态变化。激光损伤两周后，通过灌注高分子量异硫氰酸荧光素（FITC）-葡聚糖计算CNV大小。通过逆转录聚合酶链反应（RT-PCR）测量每组10只大鼠视网膜-脉络膜组织中VEGF mRNA的表达。
治疗两周后，与仅激光组相比，接受Ad-TTP注射的大鼠激光诱导的CNV面积减少了约60%。Ad-TTP注射组中VEGF mRNA表达有下降趋势。
在这个实验性激光诱导的CNV模型中，单次玻璃体内注射Ad-TTP可显著抑制CNV大小。与激光诱导的CNV组相比，Ad-TTP注射还降低了VEGF mRNA表达。本研究作为第一项研究玻璃体内注射递送锌指蛋白36的效果的研究具有重要意义。
