mRNA 在路上：走向其生物学命运的道路。
mRNA on the move: the road to its biological destiny.
机构信息
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA.
出版信息
J Biol Chem. 2013 Jul 12;288(28):20361-8. doi: 10.1074/jbc.R113.452094. Epub 2013 May 28.
Abstract
Cells have evolved to regulate the asymmetric distribution of specific mRNA targets to institute spatial and temporal control over gene expression. Over the last few decades, evidence has mounted as to the importance of localization elements in the mRNA sequence and their respective RNA-binding proteins. Live imaging methodologies have shown mechanistic details of this phenomenon. In this minireview, we focus on the advanced biochemical and cell imaging techniques used to tweeze out the finer aspects of mechanisms of mRNA movement.
摘要
细胞已经进化到能够调节特定 mRNA 靶标的不对称分布，从而对基因表达进行空间和时间上的控制。在过去的几十年中，越来越多的证据表明 mRNA 序列中的定位元件及其各自的 RNA 结合蛋白的重要性。活体成像方法学已经揭示了这种现象的机制细节。在这篇综述中，我们专注于用于提取 mRNA 运动机制的更细微方面的先进生化和细胞成像技术。
相似文献
1
mRNA on the move: the road to its biological destiny.mRNA 在路上：走向其生物学命运的道路。
J Biol Chem. 2013 Jul 12;288(28):20361-8. doi: 10.1074/jbc.R113.452094. Epub 2013 May 28.
2
A predominantly nuclear protein affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons.一种主要存在于细胞核中的蛋白质，它影响成纤维细胞和神经元中β-肌动蛋白mRNA的细胞质定位。
J Cell Biol. 2002 Jan 7;156(1):41-51. doi: 10.1083/jcb.200105133.
3
Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm.前体mRNA剪接在细胞核中用一种存在于细胞质中的新型RNA结合蛋白标记mRNA。
Mol Cell. 2000 Sep;6(3):673-82. doi: 10.1016/s1097-2765(00)00065-4.
4
Two ZBP1 KH domains facilitate beta-actin mRNA localization, granule formation, and cytoskeletal attachment.两个ZBP1 KH结构域促进β-肌动蛋白mRNA的定位、颗粒形成和细胞骨架附着。
J Cell Biol. 2003 Jan 6;160(1):77-87. doi: 10.1083/jcb.200206003. Epub 2002 Dec 30.
5
[Proteins interacting with fragments of pre-mRNA and mRNA of the human ribosomal protein S26].[与人核糖体蛋白S26的前体mRNA和mRNA片段相互作用的蛋白质]
Mol Biol (Mosk). 2000 Jan-Feb;34(1):41-7.
6
A cytoplasmic complex mediates specific mRNA recognition and localization in yeast.细胞质复合物介导酵母中特异性 mRNA 的识别和定位。
PLoS Biol. 2011 Apr;9(4):e1000611. doi: 10.1371/journal.pbio.1000611. Epub 2011 Apr 19.
7
She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae.She3p具有酿酒酵母中ASH1 mRNA定位所需的一种新活性。
Eukaryot Cell. 2009 Jul;8(7):1072-83. doi: 10.1128/EC.00084-09. Epub 2009 May 8.
8
Spatial localization of pre-mRNA transcription and processing within the nucleus.细胞核内前体mRNA转录与加工的空间定位
Curr Opin Biotechnol. 1994 Dec;5(6):579-84. doi: 10.1016/0958-1669(94)90078-7.
9
Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1.异质性核糖核蛋白A1对HIV-1依赖于Rev的未剪接mRNA向细胞质输出的协同刺激作用。
J Mol Biol. 1999 Feb 5;285(5):1951-64. doi: 10.1006/jmbi.1998.2473.
10
The polyribosomal protein bound to the 3' end of histone mRNA can function in histone pre-mRNA processing.与组蛋白mRNA 3'端结合的多核糖体蛋白可在组蛋白前体mRNA加工中发挥作用。
Nucleic Acids Symp Ser. 1995(33):234-6.
引用本文的文献
1
The dynamic world of RNA: beyond translation to subcellular localization and function.RNA的动态世界：从翻译到亚细胞定位及功能的拓展
Front Genet. 2024 Mar 12;15:1373899. doi: 10.3389/fgene.2024.1373899. eCollection 2024.
2
Enhancement of the therapeutic efficacy of mesenchymal stem cell-derived exosomes in osteoarthritis.增强间充质干细胞来源的外泌体在骨关节炎中的治疗效果。
Cell Mol Biol Lett. 2023 Sep 28;28(1):75. doi: 10.1186/s11658-023-00485-2.
3
Importance of Extracellular Vesicle Derived RNAs as Critical Colorectal Cancer Biomarkers.细胞外囊泡衍生RNA作为关键结直肠癌生物标志物的重要性。
ACS Bio Med Chem Au. 2022 Mar 1;2(3):222-235. doi: 10.1021/acsbiomedchemau.1c00043. eCollection 2022 Jun 15.
4
CEP63 upregulates YAP1 to promote colorectal cancer progression through stabilizing RNA binding protein FXR1.CEP63 通过稳定 RNA 结合蛋白 FXR1 上调 YAP1 促进结直肠癌进展。
Oncogene. 2022 Sep;41(39):4433-4445. doi: 10.1038/s41388-022-02439-y. Epub 2022 Aug 22.
5
In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG.利用 BglG 在植物体内可视化移动的 mRNA 颗粒
Methods Mol Biol. 2022;2457:411-426. doi: 10.1007/978-1-0716-2132-5_28.
6
Xist nucleates local protein gradients to propagate silencing across the X chromosome.Xist 引发局部蛋白质梯度，在 X 染色体上传播沉默。
Cell. 2021 Dec 9;184(25):6174-6192.e32. doi: 10.1016/j.cell.2021.10.022. Epub 2021 Nov 4.
7
The Great Escape: mRNA Export through the Nuclear Pore Complex.《大逃亡：通过核孔复合体的 mRNA 输出》。
Int J Mol Sci. 2021 Oct 29;22(21):11767. doi: 10.3390/ijms222111767.
8
Small extracellular vesicles with LncRNA H19 "overload": YAP Regulation as a Tendon Repair Therapeutic Tactic.携带长链非编码RNA H19“过载”的小细胞外囊泡：作为肌腱修复治疗策略的Yes相关蛋白（YAP）调控
iScience. 2021 Feb 17;24(3):102200. doi: 10.1016/j.isci.2021.102200. eCollection 2021 Mar 19.
9
Molecular mechanisms behind mRNA localization in axons.mRNA 在轴突中定位的分子机制。
Open Biol. 2020 Sep;10(9):200177. doi: 10.1098/rsob.200177. Epub 2020 Sep 23.
10
Illuminating RNA Biology: Tools for Imaging RNA in Live Mammalian Cells.揭示 RNA 生物学：在活哺乳动物细胞中成像 RNA 的工具。
Cell Chem Biol. 2020 Aug 20;27(8):891-903. doi: 10.1016/j.chembiol.2020.06.010. Epub 2020 Jul 7.
本文引用的文献
1
β-Actin mRNA compartmentalization enhances focal adhesion stability and directs cell migration.β-肌动蛋白 mRNA 的区室化增强了黏着斑的稳定性并指导细胞迁移。
Genes Dev. 2012 Sep 1;26(17):1885-90. doi: 10.1101/gad.190413.112.
2
Principles and roles of mRNA localization in animal development.mRNA 定位在动物发育中的原理和作用。
Development. 2012 Sep;139(18):3263-76. doi: 10.1242/dev.078626.
3
An unbiased analysis method to quantify mRNA localization reveals its correlation with cell motility.一种定量 mRNA 定位的无偏分析方法揭示了其与细胞迁移的相关性。
Cell Rep. 2012 Feb 23;1(2):179-84. doi: 10.1016/j.celrep.2011.12.009. Epub 2012 Feb 16.
4
Fluorescence fluctuation spectroscopy enables quantitative imaging of single mRNAs in living cells.荧光波动光谱技术可实现活细胞中单分子 mRNA 的定量成像。
Biophys J. 2012 Jun 20;102(12):2936-44. doi: 10.1016/j.bpj.2012.05.017. Epub 2012 Jun 19.
5
RNA-binding proteins involved in RNA localization and their implications in neuronal diseases.参与 RNA 定位的 RNA 结合蛋白及其在神经疾病中的意义。
Eur J Neurosci. 2012 Jun;35(12):1818-36. doi: 10.1111/j.1460-9568.2012.08160.x.
6
Nuclear export of single native mRNA molecules observed by light sheet fluorescence microscopy.利用光片荧光显微镜观察到单个天然 mRNA 分子的核输出。
Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9426-31. doi: 10.1073/pnas.1201781109. Epub 2012 May 21.
7
Arc mRNA docks precisely at the base of individual dendritic spines indicating the existence of a specialized microdomain for synapse-specific mRNA translation.Arc mRNA 精确地停靠在单个树突棘的基部，表明存在一个专门的微域，用于突触特异性 mRNA 翻译。
J Comp Neurol. 2012 Oct 1;520(14):3105-19. doi: 10.1002/cne.23073.
8
IGF2BP1 promotes cell migration by regulating MK5 and PTEN signaling.IGF2BP1 通过调节 MK5 和 PTEN 信号促进细胞迁移。
Genes Dev. 2012 Jan 15;26(2):176-89. doi: 10.1101/gad.177642.111.
9
An intracellular transmission control protocol: assembly and transport of ribonucleoprotein complexes.一种细胞内传输控制协议：核糖核蛋白复合物的组装和运输。
Curr Opin Cell Biol. 2012 Apr;24(2):202-10. doi: 10.1016/j.ceb.2011.12.014. Epub 2012 Jan 23.
10
Regulation of local expression of cell adhesion and motility-related mRNAs in breast cancer cells by IMP1/ZBP1.IMP1/ZBP1 调控乳腺癌细胞中细胞黏附和运动相关 mRNA 的局部表达。
J Cell Sci. 2012 Jan 1;125(Pt 1):81-91. doi: 10.1242/jcs.086132. Epub 2012 Jan 20.
Zotero 插件