Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA.
J Biol Chem. 2013 Jul 12;288(28):20361-8. doi: 10.1074/jbc.R113.452094. Epub 2013 May 28.
Cells have evolved to regulate the asymmetric distribution of specific mRNA targets to institute spatial and temporal control over gene expression. Over the last few decades, evidence has mounted as to the importance of localization elements in the mRNA sequence and their respective RNA-binding proteins. Live imaging methodologies have shown mechanistic details of this phenomenon. In this minireview, we focus on the advanced biochemical and cell imaging techniques used to tweeze out the finer aspects of mechanisms of mRNA movement.
细胞已经进化到能够调节特定 mRNA 靶标的不对称分布，从而对基因表达进行空间和时间上的控制。在过去的几十年中，越来越多的证据表明 mRNA 序列中的定位元件及其各自的 RNA 结合蛋白的重要性。活体成像方法学已经揭示了这种现象的机制细节。在这篇综述中，我们专注于用于提取 mRNA 运动机制的更细微方面的先进生化和细胞成像技术。
