2型糖尿病患者血清apelin-13水平升高与增殖性糖尿病视网膜病变相关。
Elevation of serum apelin-13 associated with proliferative diabetic retinopathy in type 2 diabetic patients.
作者信息
Du Jun-Hui, Li Xia, Li Rong, Xu Lin, Ma Ran-Ran, Liu Song-Fang, Zhang Zhong, Sun Hong-Zhi
机构信息
Department of Ophthalmology, Xi'an Ninth Hospital Affiliated to Medical College of Xi'an Jiaotong University, Xi'an 710054, Shaanxi Province, China ; Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
Outpatient Department, Guangzhou Sanitorium of PLA, Guangzhou 510515, Guangdong Province, China.
出版信息
Int J Ophthalmol. 2014 Dec 18;7(6):968-73. doi: 10.3980/j.issn.2222-3959.2014.06.10. eCollection 2014.
AIM
To compare apelin-13, a ligand of G-protein-coupled receptor which has been shown to be involved in retinal angiogenesis, and vascular endothelial growth factor (VEGF) serum levels in type 2 diabetes mellitus (T2DM) with or without retinopathy, and to investigate the relationship between the serum concentration of apelin-13 and diabetes retinopathy.
METHODS
Sixty-nine patients with T2DM were enrolled. Of the 69 patients, 16 had proliferative diabetic retinopathy (PDR group), 23 had non-PDR (NPDR group) and 30 had no retinopathy (T2DM group). Subjects' information, including demographics, medical history, and use of medications were recorded. Their serum samples were collected for measuring the levels of C-reactive protein (CRP), serum lipid and glycosylated hemoglobin. Apelin-13 and VEGF serum levels were measured by enzyme-linked immunosorbent assay. Kruskal-Wallis test and one-way ANOVA were used to compare the differences among these groups. Chi-square test was used to assess categorical variables. Correlations between variables were investigated by Spearman rho correlation test and stepwise regression analysis. All statistical analyses were performed through SPSS 17.0 software.
RESULTS
Sex, age, body mass index (BMI), blood pressure, CRP, hemoglobin A1c (HbA1c) have no significantly difference in the three groups. Serum level of apelin-13 was significantly elevated in PDR group as compared with T2DM group (P=0.041). Differences of VEGF serum concentration in the three groups were statistically significant (P=0.007, P=0.007 and P<0.001, respectively). Spearman rho correlation test showed that serum apelin-13 was positively correlated with BMI, serum triglycerides, VEGF, but not with age, duration of diabetes, blood pressure, CRP, HbA1c and total-cholesterol. Stepwise regression analysis showed that BMI also significantly associated with serum apelin-13 (P=0.002), while VEGF and serum triglycerides were irrelevant.
CONCLUSION
This study elucidated a positive association of apelin-13 serum level with PDR, but not with VEGF. Apelin-13 may influence the promotion of PDR but unrelated with VEGF.
目的
比较已被证明参与视网膜血管生成的G蛋白偶联受体配体apelin - 13与血管内皮生长因子（VEGF）在有或无视网膜病变的2型糖尿病（T2DM）患者中的血清水平，并探讨apelin - 13血清浓度与糖尿病视网膜病变之间的关系。
方法
纳入69例T2DM患者。69例患者中，16例患有增殖性糖尿病视网膜病变（PDR组），23例患有非增殖性糖尿病视网膜病变（NPDR组），30例无视网膜病变（T2DM组）。记录受试者的信息，包括人口统计学资料、病史和用药情况。采集他们的血清样本以测量C反应蛋白（CRP）、血脂和糖化血红蛋白水平。采用酶联免疫吸附测定法测量apelin - 13和VEGF血清水平。使用Kruskal - Wallis检验和单因素方差分析比较这些组之间的差异。采用卡方检验评估分类变量。通过Spearman秩相关检验和逐步回归分析研究变量之间的相关性。所有统计分析均通过SPSS 17.0软件进行。
结果
三组患者的性别、年龄、体重指数（BMI）、血压、CRP、糖化血红蛋白（HbA1c）无显著差异。与T2DM组相比，PDR组的apelin - 13血清水平显著升高（P = 0.041）。三组VEGF血清浓度差异有统计学意义（分别为P = 0.007、P = 0.007和P < 0.001）。Spearman秩相关检验显示，血清apelin - 13与BMI、血清甘油三酯、VEGF呈正相关，但与年龄、糖尿病病程、血压、CRP、HbA1c和总胆固醇无关。逐步回归分析显示，BMI也与血清apelin - 13显著相关（P = 0.002），而VEGF和血清甘油三酯无关。
结论
本研究阐明了apelin - 13血清水平与PDR呈正相关，但与VEGF无关。Apelin - 13可能影响PDR的进展，但与VEGF无关。
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