Hosseinimehr Seyed Jalal, Asadian Reza, Naghshvar Farshad, Azizi Soheil, Jafarinejad Mehdi, Noaparast Zohreh, Abedi Seyed Mohammad, Hosseini Seyed Amir Hossein
Department of Radiopharmacy, Faculty of Pharmacy, Traditional and Complementary Medicine Research Center, Mazandaran University of Medical Sciences , Sari , Iran .
Ren Fail. 2015 Mar;37(2):280-4. doi: 10.3109/0886022X.2014.991998. Epub 2014 Dec 26.
In this study, we investigated the protective effect of thymol as a natural compound against cisplatin-induced nephrotoxicity by quantitative renal 99mTc-DMSA uptake and compared its effect with histopathology in mice.
Mice were divided into six groups as control, cisplatin (7.5 mg/kg, intraperitoneally), thymol+cisplatin (thymol; 50 and 150 mg/kg+cisplatin; 7.5 mg/kg) and thymol (50 and 150 mg/kg). Thymol was orally administrated for two days before cisplatin injection and continued for 4 days. (99m)Tc-DMSA was injected through the tail of mice after the drug administration. The percentage of the injected dose per gram of kidney tissue (%ID/g) was calculated. In other experiment, kidneys of treated mice were assessed for histopathology.
99mTc-DMSA uptake per gram tissue of the kidneys as %ID/g was 85.27±21.81, 45.55±5.50, 65.02±32.21 and 88.46±20.46 in the control, cisplatin, thymol (50 mg/kg)+cisplatin and thymol (150 mg/kg)+cisplatin. Thymol administration with cisplatin resulted in a significant increase in the level of %ID/g. Histopathological examinations showed a protective effect of thymol against cisplatin nephrotoxicity in mice.
The results showed that thymol significantly attenuates the cisplatin-induced nephrotoxicity in mice, and 99mTc-DMSA uptake in kidney is a suitable method for assessment of nephrotoxicity in mice.
在本研究中，我们通过定量肾脏99mTc-DMSA摄取来研究天然化合物百里香酚对顺铂诱导的肾毒性的保护作用，并将其效果与小鼠组织病理学结果进行比较。
将小鼠分为六组，即对照组、顺铂组（7.5mg/kg，腹腔注射）、百里香酚+顺铂组（百里香酚；50和150mg/kg+顺铂；7.5mg/kg）以及百里香酚组（50和150mg/kg）。在注射顺铂前两天口服给予百里香酚，并持续4天。给药后通过小鼠尾部注射（99m）Tc-DMSA。计算每克肾脏组织的注射剂量百分比（%ID/g）。在另一项实验中，对处理过的小鼠肾脏进行组织病理学评估。
对照组、顺铂组、百里香酚（50mg/kg）+顺铂组和百里香酚（150mg/kg）+顺铂组每克肾脏组织的99mTc-DMSA摄取量（%ID/g）分别为85.27±21.81、45.55±5.50、65.02±32.21和88.46±20.46。与顺铂联合给予百里香酚导致%ID/g水平显著升高。组织病理学检查显示百里香酚对小鼠顺铂肾毒性具有保护作用。
结果表明，百里香酚可显著减轻小鼠顺铂诱导的肾毒性，肾脏99mTc-DMSA摄取是评估小鼠肾毒性的合适方法。
