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镁依赖的超螺旋诱导(dG)n.(dC)n片段的转变以及(dG)n链形成新的G结构。

Magnesium-dependent supercoiling-induced transition in (dG)n.(dC)n stretches and formation of a new G-structure by (dG)n strand.

作者信息

Panyutin I G, Kovalsky O I, Budowsky E I

机构信息

N.D. Zelinsky Institute of Organic Chemistry, USSR Academy of Sciences, Moscow.

出版信息

Nucleic Acids Res. 1989 Oct 25;17(20):8257-71. doi: 10.1093/nar/17.20.8257.

DOI:10.1093/nar/17.20.8257
PMID:2554253
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334962/
Abstract

Plasmids containing (dG)27.(dC)27 inserts (pPG27), (dG)37.(dC)37 inserts (pPG37), and (dG)24C(dG)21.(dC)24G(dC)21 inserts (pPG46C) were constructed for the study of structural transitions within (dG)n.(dC)n stretches. Two-dimensional gel electrophoresis has shown that a Mg2+-dependent supercoiling-induced structural transition takes place at pH 8 in plasmid pPG46C. The transition occurs at -0=0.06 and involves a supercoiling release corresponding to 5 superhelical turns. After denaturation of the restriction fragments containing (dG)n.(dC)n inserts, the strands do not renature completely and (dG)n-containing strand migrates in PAGE much faster than the (dC)n-containing one. Chemical modification experiments with the (dG)n-strand have revealed the periodic nature of the protection of guanines against dimethyl sulfate methylation. The (dG)n strand in the presence of Mg2+ forms complexes with the complementary (dC)n strand, which differ from the native duplex in mobility. We believe these effects to be due to the formation of an intrastrand structure within the (dG)n strand stabilized by G.G interactions (we called it G-structure), which in the presence of Mg2+ forms an interstrand complex. with the (dC)n strand.

摘要

构建了含有(dG)27.(dC)27插入片段（pPG27）、(dG)37.(dC)37插入片段（pPG37）和(dG)24C(dG)21.(dC)24G(dC)21插入片段（pPG46C）的质粒，用于研究(dG)n.(dC)n片段内的结构转变。二维凝胶电泳表明，在pH 8时，质粒pPG46C中发生了Mg2+依赖的超螺旋诱导的结构转变。该转变发生在-0=0.06时，涉及相当于5个超螺旋圈的超螺旋释放。含有(dG)n.(dC)n插入片段的限制性片段变性后，链不能完全复性，含(dG)n的链在聚丙烯酰胺凝胶电泳（PAGE）中的迁移速度比含(dC)n的链快得多。对(dG)n链进行的化学修饰实验揭示了鸟嘌呤对硫酸二甲酯甲基化保护的周期性。在Mg2+存在下，(dG)n链与互补的(dC)n链形成复合物，其迁移率与天然双链不同。我们认为这些效应是由于(dG)n链内通过G.G相互作用稳定的链内结构（我们称之为G结构）的形成，在Mg2+存在下，该结构与(dC)n链形成链间复合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/5ce3269c43aa/nar00137-0239-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/443f0400ba71/nar00137-0232-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/420200efd322/nar00137-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/a4f3d85b2cc9/nar00137-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/12870ca96cbf/nar00137-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/5336c254a9a7/nar00137-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/911c0408eb62/nar00137-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/ed216f201c70/nar00137-0238-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/5ce3269c43aa/nar00137-0239-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/443f0400ba71/nar00137-0232-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/420200efd322/nar00137-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/a4f3d85b2cc9/nar00137-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/12870ca96cbf/nar00137-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/5336c254a9a7/nar00137-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/911c0408eb62/nar00137-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/ed216f201c70/nar00137-0238-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1678/334962/5ce3269c43aa/nar00137-0239-a.jpg

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