Ghaffari Neda, Parry Samuel, Elovitz Michal A, Durnwald Celeste P
Department of Obstetrics and Gynecology, Maternal and Child Health Research Program, Center for Research on Reproduction and Women's Health, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, United States
Department of Obstetrics and Gynecology, Maternal and Child Health Research Program, Center for Research on Reproduction and Women's Health, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, United States.
Reprod Sci. 2015 Jul;22(7):860-4. doi: 10.1177/1933719114565032. Epub 2014 Dec 28.
Exposure to maternal obesity in utero predisposes offspring to obesity and metabolic disease. This study investigated whether maternal obesity is associated with alterations in expression of fetal microRNA (miRNA).
A cohort study of women with body mass index (BMI) ≥35 kg/m(2) (n = 16) versus those with normal BMI 20 to 24.9 (n = 20) was performed. All participants had normal glucose tolerance (1-hour glucose challenge test <130) and normally grown neonates (2700-3500 g). Umbilical cord samples were collected immediately after delivery. Expression of miRNA was assessed using Affymetrix GeneChip miRNA 3.0 Arrays. Differential miRNA expression was determined using Student t tests with Benjamini-Hocherg correction.
For 1733 human mature miRNAs, the expression levels were not statistically different in umbilical cord blood samples from pregnancies of obese women compared to controls.
Expression of fetal miRNA is not altered in umbilical cord blood in response to in utero exposure to obesity. Alternate mechanisms underlying the fetal effects of maternal obesity should be explored.
子宫内暴露于母体肥胖会使后代易患肥胖症和代谢性疾病。本研究调查了母体肥胖是否与胎儿微小RNA（miRNA）表达的改变有关。
对体重指数（BMI）≥35 kg/m²的女性（n = 16）与BMI正常（20至24.9）的女性（n = 20）进行了一项队列研究。所有参与者糖耐量正常（1小时葡萄糖耐量试验<130）且新生儿生长正常（2700 - 3500 g）。分娩后立即采集脐带样本。使用Affymetrix GeneChip miRNA 3.0阵列评估miRNA的表达。采用经Benjamini-Hochberg校正的Student t检验确定miRNA表达差异。
对于1733种人类成熟miRNA，肥胖女性妊娠的脐带血样本与对照组相比，其表达水平无统计学差异。
子宫内暴露于肥胖并不会使脐带血中胎儿miRNA的表达发生改变。应探索母体肥胖对胎儿影响的其他潜在机制。
