Zargar Ruby, Urwat Uneeb, Malik Firdose, Shah Riaz A, Bhat M H, Naykoo Niyaz A, Khan Firdous, Khan H M, Ahmed Syed Mudasir, Vijh Ramesh Kumar, Ganai Nazir A
Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
Res Vet Sci. 2015 Feb;98:51-8. doi: 10.1016/j.rvsc.2014.11.016. Epub 2014 Dec 11.
Pashmina goat inhabits the high altitude cold arid desert of Ladakh, India. This goat is known for its finest and costliest under fiber. Though the under fiber may be a part of its complex thermoregulation mechanism, the genetics of its adaptability under cold conditions is not known. As an attempt to understand its adaptive genetics, and the role of RNA-binding proteins at the cellular response, this study was conducted to characterize the RBM3 gene in Pashmina goat and its expression during hypothermia. The ORF of Pashmina RBM3 gene was 273 bp. Phylogenetic analysis revealed that Pashmina RBM3 is closely related to Bos taurus RBM3. Pashmina RBM3 was characterized by comparative modeling studies. The final 3-D model contained two α-helices and four β-sheets. qRT-PCR data showed that Pashmina RBM3 gene expression was significantly higher (P < 0.05) at moderate (30 °C) hypothermic stress conditions as compared with deep (15 °C) hypothermia.
克什米尔山羊栖息在印度拉达克高海拔寒冷干旱的沙漠地区。这种山羊以其最优质、最昂贵的底层绒毛而闻名。尽管底层绒毛可能是其复杂体温调节机制的一部分，但其在寒冷条件下适应性的遗传学尚不清楚。为了试图了解其适应性遗传学以及RNA结合蛋白在细胞反应中的作用，本研究旨在对克什米尔山羊的RBM3基因进行表征，并研究其在体温过低时的表达情况。克什米尔山羊RBM3基因的开放阅读框为273 bp。系统发育分析表明，克什米尔山羊RBM3与牛的RBM3密切相关。通过比较建模研究对克什米尔山羊RBM3进行了表征。最终的三维模型包含两个α螺旋和四个β折叠。实时定量PCR数据显示，与深度（15°C）低温相比，在中度（30°C）低温应激条件下，克什米尔山羊RBM3基因的表达显著更高（P<0.05）。
