Biochemistry Department, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou 550004, China.
Guiyang Medical College, Guiyang, Guizhou 550004, China.
Oxid Med Cell Longev. 2014;2014:469059. doi: 10.1155/2014/469059. Epub 2014 Nov 27.
NAFLD model rats were established and divided into NAFLD model (MG group), SIRT1 RNAi (SI group), blueberry juice (BJ group), blueberry juice + bifidobacteria (BJB group), blueberry juice + SIRT1 RNAi (BJSI group), and blueberry juice + bifidobacteria + SIRT1 RNAi groups (BJBSI group). A group with normal rats was a control group (CG). BJB group ameliorated NAFLD, which was better than BJ group (P < 0.05). The lipid accumulation was lower in CG, BJ, and BJB groups than that in MG, SI, BJSI, and BJBSI groups (P < 0.05). The levels of SIRT1 and PPAR-α were higher in CG, BJ, and BJB groups than those in MG, SI, BJSI, and BJBSI groups (P < 0.05). The levels of SREBP-1c were lower in CG, BJ, and BJB groups than those in MG, SI, BJSI, and BJBSI groups (P < 0.05). The biochemical indexes SOD, GSH, and HDL-c were improved from CG to BJB group (P < 0.05). Inversely, the levels of AST and ALT, TG, TC, LDL-c, and MDA were decreased from CG to BJB group (P < 0.05). These changes enhance antioxidative capability and biochemical index of rats. Blueberry juice and bifidobacteria improve NAFLD by activating SIRTI-mediating signaling pathway.
非酒精性脂肪性肝病模型大鼠被建立并分为非酒精性脂肪性肝病模型（MG 组）、SIRT1 RNAi（SI 组）、蓝莓汁（BJ 组）、蓝莓汁+双歧杆菌（BJB 组）、蓝莓汁+SIRT1 RNAi（BJSI 组）和蓝莓汁+双歧杆菌+SIRT1 RNAi 组（BJBSI 组）。一组正常大鼠为对照组（CG）。BJB 组改善了非酒精性脂肪性肝病，优于 BJ 组（P<0.05）。CG、BJ 和 BJB 组的脂质积累低于 MG、SI、BJSI 和 BJBSI 组（P<0.05）。CG、BJ 和 BJB 组的 SIRT1 和 PPAR-α 水平高于 MG、SI、BJSI 和 BJBSI 组（P<0.05）。CG、BJ 和 BJB 组的 SREBP-1c 水平低于 MG、SI、BJSI 和 BJBSI 组（P<0.05）。从 CG 到 BJB 组，生化指标 SOD、GSH 和 HDL-c 得到改善（P<0.05）。相反，从 CG 到 BJB 组，AST 和 ALT、TG、TC、LDL-c 和 MDA 的水平降低（P<0.05）。这些变化增强了大鼠的抗氧化能力和生化指标。蓝莓汁和双歧杆菌通过激活 SIRTI 介导的信号通路改善非酒精性脂肪性肝病。
