Mandl C W, Heinz F X, Stöckl E, Kunz C
Institute of Virology, University of Vienna, Austria.
Virology. 1989 Nov;173(1):291-301. doi: 10.1016/0042-6822(89)90246-8.
The genome sequence of tick-borne encephalitis (TBE) virus (Western subtype vaccine strain Neudoerfl) was determined. This extends the previously published sequence of the structural proteins to the nonstructural protein region and noncoding sequences at the 5'- and 3'-termini. The amino-termini of the individual proteins were assigned by comparison with other flavivirus sequences. Amino acid homology calculations between TBE virus and mosquito-borne flaviviruses were performed for all nonstructural proteins. An evolutionary tree based on protein NS1 is presented that reveals the molecular basis of relationships among flaviviruses. Tick-borne and mosquito-borne flaviviruses share a common hydrophilicity profile and also other features of their primary sequences, such as the presumably functional Gly-Asp-Asp sequence element within protein NS5. Other characteristics, such as the potential N-glycosylation sites of protein NS1 and a potential proteolytic cleavage site within protein NS4B, are conserved within the mosquito-borne group, but differ in the TBE virus sequence.
确定了蜱传脑炎（TBE）病毒（西方亚型疫苗株Neudoerfl）的基因组序列。这将先前公布的结构蛋白序列扩展至非结构蛋白区域以及5'和3'末端的非编码序列。通过与其他黄病毒序列比较确定了各个蛋白的氨基末端。对所有非结构蛋白进行了TBE病毒与蚊传黄病毒之间的氨基酸同源性计算。给出了基于NS1蛋白的进化树，揭示了黄病毒之间关系的分子基础。蜱传和蚊传黄病毒具有共同的亲水性图谱以及其一级序列的其他特征，例如NS5蛋白内推测具有功能的甘氨酸-天冬氨酸-天冬氨酸序列元件。其他特征，如NS1蛋白的潜在N-糖基化位点和NS4B蛋白内的潜在蛋白水解切割位点，在蚊传病毒组内是保守的，但在TBE病毒序列中有所不同。
