Mandl C W, Heinz F X, Kunz C
Institute of Virology, University of Vienna, Austria.
Virology. 1988 Sep;166(1):197-205. doi: 10.1016/0042-6822(88)90161-4.
Tick-borne encephalitis (TBE) virus (Western subtype strain Neudoerfl) was cloned and the sequence of 2450 nucleotides of the 5'-terminal region of the genome was determined. By amino acid sequencing and sequence comparisons with other flaviviruses the amino-termini of the structural proteins and protein NS1 were localized. Sequence homologies with other flaviviruses were determined and corresponded well to the established serological classification system of the Flavivirus family. N-Glycosylation sites were found to be conserved to a large extent among members of the same serological subgroup, but not between members of different subgroups. Hydrophilicity plots and sequence comparisons revealed that the TBE virus capsid protein exhibited features distinct from all other flaviviruses. Additionally, the capsid protein coding region of another natural isolate of TBE virus (strain ZZ-9) was sequenced in order to analyze why the capsid protein of this strain exhibited a significantly faster migration rate on SDS-polyacrylamide gels than other TBE virus strains.
蜱传脑炎（TBE）病毒（西方亚型纽德奥弗尔毒株）被克隆，并测定了基因组5'末端区域2450个核苷酸的序列。通过氨基酸测序以及与其他黄病毒的序列比较，确定了结构蛋白和NS1蛋白的氨基末端。测定了与其他黄病毒的序列同源性，其与黄病毒科既定的血清学分类系统高度吻合。发现N - 糖基化位点在同一血清学亚组的成员中在很大程度上是保守的，但在不同亚组的成员之间则不然。亲水性图谱和序列比较表明，TBE病毒衣壳蛋白表现出与所有其他黄病毒不同的特征。此外，对TBE病毒另一个自然分离株（ZZ - 9株）的衣壳蛋白编码区进行了测序，以分析该毒株的衣壳蛋白在SDS - 聚丙烯酰胺凝胶上迁移速度为何明显快于其他TBE病毒株。
