Di Luca D, Caselli E, Monini P, Rotola A, Savioli A, Cassai E
Istituto di Microbiologia, Università di Ferrara, Italy.
Virus Res. 1989 Sep;14(1):49-55. doi: 10.1016/0168-1702(89)90068-3.
An invasive cervical carcinoma was found to harbor an episomal variant of human papillomavirus (HPV) type 16 DNA, with a size of about 10.1 kb. A genomic library of the tumor was constructed in bacteriophage lambda and a recombinant phage clone was isolated by screening with HPV 16 probe. Analysis by restriction mapping and Southern hybridization showed that the isolate contained a 2.2 kb duplication of the early region, which included part of E6, all E7 and part of E1 open reading frames. Possible consequences of this duplication for oncogenesis are discussed.
发现一例浸润性宫颈癌含有16型人乳头瘤病毒（HPV）的游离型DNA变异体，大小约为10.1 kb。用噬菌体λ构建了该肿瘤的基因组文库，并通过用HPV 16探针筛选分离出一个重组噬菌体克隆。通过限制性酶切图谱分析和Southern杂交表明，分离株包含早期区域的一个2.2 kb重复片段，其中包括部分E6、全部E7和部分E1开放阅读框。讨论了这种重复对肿瘤发生的可能影响。
