Matsukura T, Sugase M
Department of Enteroviruses, National Institute of Health, Tokyo, Japan.
Virology. 1990 Aug;177(2):833-6. doi: 10.1016/0042-6822(90)90560-e.
A novel human papillomavirus type (HPV) was cloned from an invasive cervical carcinoma. The viral clone showed no homology with other known prototypes of HPV (HPV-1 through HPV-57), except HPV-33 by Southern blot analysis under stringent conditions. It showed less than 20% homology to HPV-33 by reassociation kinetic analysis. The restriction endonuclease map of the clone was different from those of other HPV types and its predicted genome organization surmised by hybridization with subgenomic fragment probes of HPV-33 DNA showed the typical HPV genome organization. The results indicate that this clone is a new type of HPV, designated as HPV-58, distinct from the other known types of HPV. HPV-58 was detected in none of 6 specimens of cervical condylomata acuminata, in 7 of 58 specimens of cervical intraepithelial neoplasia, and in 4 of 50 specimens of invasive cervical carcinoma studied in Nagano prefecture, Japan.
从一例浸润性宫颈癌中克隆出一种新型人乳头瘤病毒（HPV）。在严格条件下进行的Southern印迹分析显示，该病毒克隆与其他已知的HPV原型（HPV-1至HPV-57）无同源性，但与HPV-33有同源性。通过重缔合动力学分析，它与HPV-33的同源性低于20%。该克隆的限制性内切酶图谱与其他HPV类型不同，通过与HPV-33 DNA的亚基因组片段探针杂交推测的其预测基因组结构显示出典型的HPV基因组结构。结果表明，该克隆是一种新型HPV，命名为HPV-58，与其他已知的HPV类型不同。在日本长野县研究的6例尖锐湿疣标本、58例宫颈上皮内瘤变标本中的7例以及50例浸润性宫颈癌标本中的4例中均未检测到HPV-58。
