Mann Adrien, Thompson Allie, Robbins Nathan, Blomkalns Andra L
Department of Emergency Medicine, University of Cincinnati College of Medicine.
Department of Emergency Medicine, University of Cincinnati College of Medicine;
J Vis Exp. 2014 Dec 4(94):52174. doi: 10.3791/52174.
Obesity has increased dramatically in the last few decades and affects over one third of the adult US population. The economic effect of obesity in 2005 reached a staggering sum of $190.2 billion in direct medical costs alone. Obesity is a major risk factor for a wide host of diseases. Historically, little was known regarding adipose and its major and essential functions in the body. Brown and white adipose are the two main types of adipose but current literature has identified a new type of fat called brite or beige adipose. Research has shown that adipose depots have specific metabolic profiles and certain depots allow for a propensity for obesity and other related disorders. The goal of this protocol is to provide researchers the capacity to identify and excise adipose depots that will allow for the analysis of different factorial effects on adipose; as well as the beneficial or detrimental role adipose plays in disease and overall health. Isolation and excision of adipose depots allows investigators to look at gross morphological changes as well as histological changes. The adipose isolated can also be used for molecular studies to evaluate transcriptional and translational change or for in vitro experimentation to discover targets of interest and mechanisms of action. This technique is superior to other published techniques due to the design allowing for isolation of multiple depots with simplicity and minimal contamination.
在过去几十年中，肥胖现象急剧增加，影响了超过三分之一的美国成年人口。仅在2005年，肥胖造成的经济影响就仅直接医疗费用一项就达到了惊人的1902亿美元。肥胖是引发多种疾病的主要风险因素。从历史上看，人们对脂肪及其在体内的主要和基本功能知之甚少。棕色脂肪和白色脂肪是两种主要的脂肪类型，但当前的文献已经确定了一种新型脂肪，称为“亮”或米色脂肪。研究表明，脂肪库具有特定的代谢特征，某些脂肪库会导致肥胖和其他相关疾病的倾向。本方案的目标是为研究人员提供识别和切除脂肪库的能力，以便分析对脂肪的不同因素影响；以及脂肪在疾病和整体健康中所起的有益或有害作用。脂肪库的分离和切除使研究人员能够观察大体形态变化以及组织学变化。分离出的脂肪还可用于分子研究，以评估转录和翻译变化，或用于体外实验，以发现感兴趣的靶点和作用机制。由于该设计允许简单且污染最小地分离多个脂肪库，因此该技术优于其他已发表的技术。
