Roque Katharine, Shin Kyung-Min, Jo Ji-Hoon, Kim Hyoung-Ah, Heo Yong
Department of Occupational Health, College of Medical and Public Health Sciences, Catholic University of Daegu, Gyeongsan 712-702, Korea.
J Vet Sci. 2015;16(2):173-7. doi: 10.4142/jvs.2015.16.2.173. Epub 2014 Dec 24.
Hazardous biochemical agents in animal husbandry indoor environments are known to promote the occurrence of various illnesses among workers and animals. The relationship between endotoxin levels in dust collected from chicken farms and various immunological markers was investigated. Peripheral blood was obtained from 20 broiler chickens and 20 laying hens from four different chicken farms in Korea. Concentrations of total or respirable dust in the inside the chicken farm buildings were measured using a polyvinyl chloride membrane filter and mini volume sampler. Endotoxin levels in the dust were determined by the Limulus Amebocyte Lysate Kinetic method. Interferon-γ production by peripheral blood mononuclear cells stimulated with concanavalin A was significantly lower in broilers or layers from the farms with higher endotoxin concentrations than the chickens from the farms with lower endotoxin levels. An opposite pattern was observed for plasma cortisol concentrations with higher cortisol levels found in chickens from the farms with higher endotoxin levels. When peripheral lymphocytes were examined, the percentage of CD3(-)Ia(+) B cells was lower in layers from farms with higher endotoxin levels than those from locations with lower endotoxin levels. Overall, these results suggest a probable negative association between dust endotoxin levels and cell-mediated immunity in chickens.
众所周知，畜牧室内环境中的有害生化制剂会促使工人和动物患上各种疾病。研究了从养鸡场收集的灰尘中内毒素水平与各种免疫标志物之间的关系。从韩国四个不同养鸡场的20只肉鸡和20只蛋鸡采集外周血。使用聚氯乙烯膜过滤器和小型采样器测量养鸡场建筑物内总灰尘或可吸入灰尘的浓度。通过鲎试剂动力学方法测定灰尘中的内毒素水平。用伴刀豆球蛋白A刺激外周血单个核细胞产生的干扰素-γ在来自内毒素浓度较高农场的肉鸡或蛋鸡中显著低于来自内毒素水平较低农场的鸡。对于血浆皮质醇浓度，观察到相反的模式，在内毒素水平较高农场的鸡中发现皮质醇水平较高。当检查外周淋巴细胞时，来自内毒素水平较高农场的蛋鸡中CD3(-)Ia(+)B细胞的百分比低于来自内毒素水平较低地点的蛋鸡。总体而言，这些结果表明鸡的灰尘内毒素水平与细胞介导免疫之间可能存在负相关。
