Al'khovskiĭ S V, L'vov D K, Shchelkanov M Iu, Shchetinin A M, Deriabin P G, Gitel'man A K, Aristova V A, Botikov A G
Vopr Virusol. 2014 Jul-Aug;59(4):25-30.
Near full-genome sequence of the Wad Medani Virus (WMV) (strain LEIV-8066Tur) (Orbivirus, Reoviridae) isolated from the ticks Hyalomma asiaticum Schulze et Schlottke, 1929, collected from sheep in Baharly district in Turkmenistan, was determined using next generation sequencing approach. The similarity of the RNA-dependent RNA-polymerase (Pol, VP1) amino acid sequence between WMV and the Kemerovo group orbiviruses (KEMV), as well as of the Baku virus (BAKV), was 64%. The similarity of the conserved structural protein VP3 (T2) of WMV with mosquito-borne and tick-borne orbiviruses reaches 46% and 67%, respectively. For the surface proteins VP2, VP5, and VP7 (T13), which have major antigenic determinants of orbiviruses, the similarity of WMV with tickborne orbiviruses (KEMV and BAKV) is 26-30%, 45% and, 57%, respectively (ID GenBank: KJ425426-35).
采用下一代测序方法，测定了从土库曼斯坦巴哈尔利地区绵羊身上采集的亚洲璃眼蜱（Hyalomma asiaticum Schulze et Schlottke，1929）中分离出的瓦德迈达尼病毒（WMV）（毒株LEIV - 8066Tur）（环状病毒属，呼肠孤病毒科）的近全基因组序列。WMV与克麦罗沃组环状病毒（KEMV）以及巴库病毒（BAKV）之间的RNA依赖RNA聚合酶（Pol，VP1）氨基酸序列相似度为64%。WMV保守结构蛋白VP3（T2）与蚊传和蜱传环状病毒的相似度分别达到46%和67%。对于具有环状病毒主要抗原决定簇的表面蛋白VP2、VP5和VP7（T13），WMV与蜱传环状病毒（KEMV和BAKV）的相似度分别为26 - 30%、45%和57%（ID GenBank：KJ425426 - 35）。
