L'vov D K, Al'khovskiĭ S V, Shchelkanov M Iu, Shchetinin A M, Deriabin P G, Gitel'man A K, Aristova V A, Botikov A G
Vopr Virusol. 2014 Jul-Aug;59(4):15-9.
The Syr-Darya valley fever virus (SDVFV) was originally isolated from the blood of the patient with fever in the Kyzylorda province, Kazakhstan, in July 1973 and was classified to the Cardiovirus genus (fam. Picornaviridae). Later, SDVFV was isolated from the ticks Hyalomma as. asiaticum Schulze et Schlottke, 1929 (Hyalomminae) (1 strain) and Dermacentor daghestanicus Olenev, 1929 (Rhipicephalinae) (7 strains), collected in the floodplains of the Syr-Darya river and the Ili river. In this paper, complet genome of the SDVFV (strain LEIV-Tur2833) was sequenced using the next-generation sequencing approach (GenBank ID: KJ191558). It was demonstrated that, phylogenetically, the SDVFV is closely related closest to the Theiler's murine encephalomyelitis virus (TMEV) and Vilyuisk human encephalomyelitis virus (VMEV). The similarity of the SDVFV with VHEV and TMEV based on P1 region of the polyprotein-precursor (structural proteins VP1-VP4), reaches 75% and 91% for nucleotide sequences and 80% and 93% for putative amino acid sequences, respectively. For nonstructural proteins regions P2 (2A-2C) and P3 (3A-3D) similarity of SDVFV with TMEV and VHEV is 96%-98%.
锡尔河河谷热病毒（SDVFV）最初于1973年7月从哈萨克斯坦克孜勒奥尔达州一名发热患者的血液中分离出来，并被归类于心病毒属（微小核糖核酸病毒科）。后来，在锡尔河和伊犁河的河漫滩采集到的亚洲璃眼蜱（Hyalomminae）（1株）和达吉斯坦革蜱（Rhipicephalinae）（7株）中分离出了SDVFV。在本文中，使用下一代测序方法对SDVFV（毒株LEIV-Tur2833）的完整基因组进行了测序（GenBank登录号：KJ191558）。结果表明，在系统发育上，SDVFV与泰勒氏鼠脑脊髓炎病毒（TMEV）和维柳伊斯克人脑脊髓炎病毒（VMEV）关系最为密切。基于多蛋白前体的P1区域（结构蛋白VP1-VP4），SDVFV与VHEV和TMEV的核苷酸序列相似性分别达到75%和91%，推测氨基酸序列相似性分别达到80%和93%。对于非结构蛋白区域P2（2A-2C）和P3（3A-3D），SDVFV与TMEV和VHEV的相似性为96%-98%。
