Section of Occupational Medicine, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, I-60126 Ancona, Italy.
Department of Specialistic Clinical and Odontostomatological Sciences, Polytechnic University of Marche, I-60131 Ancona, Italy.
Int J Mol Med. 2021 Apr;47(4). doi: 10.3892/ijmm.2021.4881. Epub 2021 Feb 12.
New approaches are being studied for the treatment of skin cancer. It has been reported that light combined with cisplatinum may be effective against skin cancer. In the present study, the effects of specific light radiations and cisplatinum on A431 cutaneous squamous cell carcinoma (cSCC) and HaCaT non‑tumorigenic cell lines were investigated. Both cell lines were exposed to blue and red light sources for 3 days prior to cisplatinum treatment. Viability, apoptosis, cell cycle progression and apoptotic‑related protein expression levels were investigated. The present results highlighted that combined treatment with blue light and cisplatinum was more effective in reducing cell viability compared with single treatments. Specifically, an increase in the apoptotic rate was observed when the cells were treated with blue light and cisplatinum, as compared to treatment with blue light or cisplatinum alone. Combined treatment with blue light and cisplatinum also caused cell cycle arrest at the S phase. Treatment with cisplatinum following light exposure induced the expression of apoptotic proteins in the A431 and HaCaT cell lines, which tended to follow different apoptotic mechanisms. On the whole, these data indicate that blue light combined with cisplatinum may be a promising treatment for cSCC.
正在研究新的方法来治疗皮肤癌。据报道，光与顺铂联合使用可能对皮肤癌有效。在本研究中，研究了特定的光辐射和顺铂对 A431 皮肤鳞状细胞癌（cSCC）和 HaCaT 非肿瘤细胞系的影响。在顺铂治疗前，这两种细胞系均接受蓝、红光照射 3 天。检测了细胞活力、细胞凋亡、细胞周期进程和凋亡相关蛋白的表达水平。本研究结果表明，与单独治疗相比，蓝光照与顺铂联合治疗能更有效地降低细胞活力。具体而言，与单独使用蓝光照或顺铂相比，用蓝光照与顺铂联合处理时，观察到凋亡率增加。蓝光照与顺铂联合治疗还导致细胞周期在 S 期停滞。光暴露后用顺铂处理诱导 A431 和 HaCaT 细胞系中凋亡蛋白的表达，这些凋亡机制似乎不同。总的来说，这些数据表明，蓝光照联合顺铂可能是治疗 cSCC 的一种有前途的方法。
