Zimber-Strobl U, Suentzenich K O, Laux G, Eick D, Cordier M, Calender A, Billaud M, Lenoir G M, Bornkamm G W
Institut für Klinische Molekularbiologie und Tumorgenetik, Hämatologikum der GSF, Munich, Federal Republic of Germany.
J Virol. 1991 Jan;65(1):415-23. doi: 10.1128/JVI.65.1.415-423.1991.
Transcription of the terminal protein (TP) gene of Epstein-Barr virus (EBV) in Burkitt's lymphoma cells, in EBV-negative Burkitt's lymphoma cells converted with transformation-defective (P3HR1) and transformation-competent (B95-8, AG876) EBV strains, and in EBV-immortalized cell lines was studied. A TP1 cDNA probe spanning the boundary between exons 1 and 2 and discriminating between TP1 and TP2 transcripts was used for S1 analysis. TP RNA expression varied widely in Burkitt's lymphoma cells. TP-specific transcripts were not detectable or only hardly detectable in Burkitt's lymphoma cells with the group I phenotype (CD10+ CD77+ CD21- CD23- CD30- CDw70-) as well as in P3HR1 virus-converted Burkitt's lymphoma lines. TP expression was high in Burkitt's lymphoma lines with the group II and group III phenotypes (CD21+ CD23+ CD30+ CDw70+), in B95-8 and AG876 virus-converted lines, and in EBV-immortalized cells. Detection of TP1 RNA correlated with EBNA2 expression. TP1 transcription was shown to be dependent on EBNA2 expression by stable transfection of an EBNA2 expression vector into P3HR1 virus-converted BL41 cells. EBNA2 is activating the TP1 as well as the TP2 promoter, as shown by the analysis of TP promoter-chloramphenicol acetyltransferase constructs transiently transfected into EBNA2-positive and EBNA2-negative Burkitt's lymphoma cells.
对伯基特淋巴瘤细胞、用转化缺陷型（P3HR1）和转化能力型（B95 - 8、AG876）EB病毒株转化的EBV阴性伯基特淋巴瘤细胞以及EBV永生化细胞系中EB病毒（EBV）末端蛋白（TP）基因的转录进行了研究。使用跨越外显子1和2边界并区分TP1和TP2转录本的TP1 cDNA探针进行S1分析。TP RNA表达在伯基特淋巴瘤细胞中差异很大。在具有I组表型（CD10 + CD77 + CD21 - CD23 - CD30 - CDw70 -）的伯基特淋巴瘤细胞以及P3HR1病毒转化的伯基特淋巴瘤细胞系中，未检测到或仅很难检测到TP特异性转录本。在具有II组和III组表型（CD21 + CD23 + CD30 + CDw70 +）的伯基特淋巴瘤细胞系、B95 - 8和AG876病毒转化的细胞系以及EBV永生化细胞中，TP表达很高。TP1 RNA的检测与EBNA2表达相关。通过将EBNA2表达载体稳定转染到P3HR1病毒转化的BL41细胞中，表明TP1转录依赖于EBNA2表达。如对瞬时转染到EBNA2阳性和EBNA2阴性伯基特淋巴瘤细胞中的TP启动子 - 氯霉素乙酰转移酶构建体的分析所示，EBNA2激活TP1以及TP2启动子。
