Lörincz A T, Quinn A P, Goldsborough M D, McAllister P, Temple G F
Molecular Diagnostics Research, Life Technologies, Inc., Gaithersburg, Maryland 20877.
J Gen Virol. 1989 Nov;70 ( Pt 11):3099-104. doi: 10.1099/0022-1317-70-11-3099.
A new human papillomavirus type (HPV-56) was identified by low stringency Southern blot analysis with an HPV-31 DNA probe, in a cervical intraepithelial neoplasm (CIN). The DNA of this virus was molecularly cloned and shown to be a new HPV type based on the absence of cross-reactivity to HPV types 1 to 55 under high-stringency hybridization conditions. At low stringency, HPV-56 was most related to HPV types 30 and 45. The deduced organization of the open reading frames of HPV-56, from hybridization and partial nucleotide sequence analyses, reveals a typical HPV genome. HPV-56 was detected in two of 464 normal cervical tissues, in five of 227 cervical condylomas and CIN, and in two of 84 invasive cancers of the cervix.
利用人乳头瘤病毒31型（HPV-31）DNA探针，通过低严谨度的Southern印迹分析，在一例宫颈上皮内瘤变（CIN）中鉴定出一种新型人乳头瘤病毒（HPV-56）。该病毒的DNA经分子克隆，并在高严谨度杂交条件下与1至55型HPV无交叉反应，表明它是一种新型HPV。在低严谨度条件下，HPV-56与30型和45型HPV关系最为密切。通过杂交和部分核苷酸序列分析推导得出的HPV-56开放阅读框结构，显示其具有典型的HPV基因组。在464份正常宫颈组织中有2份检测到HPV-56，在227份宫颈湿疣和CIN中有5份检测到，在84份宫颈浸润癌中有2份检测到。
