Shimoda K, Lorincz A T, Temple G F, Lancaster W D
Department of Obstetrics and Gynecology, Vincent T. Lombardi Cancer Research Center, Georgetown University Medical Center, Washington, D.C. 20007.
J Gen Virol. 1988 Nov;69 ( Pt 11):2925-8. doi: 10.1099/0022-1317-69-11-2925.
Analysis of biopsies of cervical intraepithelial neoplasia (CIN) revealed a high percentage of human papillomavirus (HPV) sequences that would hybridize to a mixture of HPV probes only under conditions of relaxed stringency. The DNA sequences of one of these viruses was molecularly cloned and shown to be a new HPV, type 52 (HPV-52). This virus is most closely related to HPV-33. Hybridization analysis with restriction fragments of HPV-52 showed collinearity with the HPV-33 genome. DNA sequencing revealed a high level of conservation between the two viruses within the L1 open reading frame but significant divergence in the non-coding region of the viral genomes. Prevalence studies indicated that HPV-52 sequences were present in three of 137 (2%) CIN and in one of 48 (2%) cervical squamous cell cancers studied in the U.S.A.
对宫颈上皮内瘤变（CIN）活检样本的分析显示，只有在宽松的严谨性条件下，才有高比例的人乳头瘤病毒（HPV）序列能与HPV探针混合物杂交。其中一种病毒的DNA序列经分子克隆后显示为一种新的HPV，即52型（HPV - 52）。这种病毒与HPV - 33关系最为密切。用HPV - 52的限制性片段进行杂交分析表明，其与HPV - 33基因组具有共线性。DNA测序显示，两种病毒在L1开放阅读框内具有高度保守性，但在病毒基因组的非编码区存在显著差异。患病率研究表明，在美国研究的137例CIN中有3例（2%）以及48例宫颈鳞状细胞癌中有1例（2%）存在HPV - 52序列。
