Hardy John G, Lin Phillip, Schmidt Christine E
a J. Crayton Pruitt Family Department of Biomedical Engineering , University of Florida , Biomedical Sciences Building JG-53, P.O. Box 116131, Gainesville , FL 32611-6131 , USA.
J Biomater Sci Polym Ed. 2015;26(3):143-61. doi: 10.1080/09205063.2014.975393.
In situ crosslinking hydrogels are attractive for application as injectable hydrogel-based tissue scaffolds that adapt to fill patient-specific cavities. Oxime click chemistry was used to crosslink hydrogels that were biodegradable, soft and supportive of cell adhesion. Linear poly(ethylene glycol)s (PEGs, Mn 2 or 4 kDa) terminated at both ends with aminooxy moieties and hyaluronic acid (HA, Mn 2 MDa) derivatives displaying aldehydes were non-toxic towards primary Schwann cells. The PEG and HA derivatives form oxime crosslinked hydrogels with mechanical and swelling properties that were tunable based on the composition of the hydrogels to values analogous to soft tissues such as those found in the central or peripheral nervous system. Gels incorporating collagen-1 supported the adhesion of human mesenchymal stem cells. Such chemistry has the potential to generate clinically relevant injectable hydrogels for minimally invasive personalized medical procedures in the central or peripheral nervous systems.
原位交联水凝胶作为可注射的基于水凝胶的组织支架具有吸引力，可适应填充患者特定的腔隙。肟点击化学用于交联可生物降解、柔软且支持细胞黏附的水凝胶。两端带有氨氧基部分的线性聚乙二醇（PEG，Mn 2或4 kDa）和显示醛基的透明质酸（HA，Mn 2 MDa）衍生物对原代雪旺细胞无毒。PEG和HA衍生物形成具有机械性能和溶胀性能的肟交联水凝胶，这些性能可根据水凝胶的组成调节至类似于中枢或外周神经系统中发现的软组织的值。掺入胶原蛋白-1的凝胶支持人间充质干细胞的黏附。这种化学方法有潜力生成临床上相关的可注射水凝胶，用于中枢或外周神经系统的微创个性化医疗程序。
