College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea.
College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea ; Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, 4072, Australia .
Asian-Australas J Anim Sci. 2015 Feb;28(2):151-7. doi: 10.5713/ajas.14.0529.
Fatness qualities in pigs measured by the amount of fat deposition and composition of fatty acids (FAs) in pork have considerable effect on current breeding goals. The stearoyl-CoA desaturase (SCD) gene plays a crucial role in the conversion of saturated FAs into monounsaturated FAs (MUFAs), and hence, is among the candidate genes responsible for pig fatness traits. Here, we identified a single nucleotide polymorphism (SNP, c.*2041T>C) in the 3' untranslated region by direct sequencing focused on coding and regulatory regions of porcine SCD. According to the association analysis using a hundred of Berkshire pigs, the SNP was significantly associated with FA composition (MUFAs and polyunsaturated FAs [PUFAs]), polyunsaturated to saturated (P:S) FA ratio, n-6:n-3 FA ratio, and extent of fat deposition such as intramuscular fat and marbling (p<0.05). In addition, the SNP showed a significant effect on the SCD mRNA expression levels (p = 0.041). Based on our results, we suggest that the SCD c.*2041T>C SNP plays a role in the gene regulation and affects the fatness qualities in Berkshire pigs.
猪的肥胖特性可以通过体脂沉积量和猪肉中脂肪酸（FA）组成来衡量，这些特性对当前的育种目标有重要影响。硬脂酰辅酶 A 去饱和酶（SCD）基因在将饱和 FA 转化为单不饱和 FA（MUFAs）的过程中起着关键作用，因此是负责猪肥胖特性的候选基因之一。在这里，我们通过直接测序法在编码区和调控区对猪 SCD 进行了集中研究，鉴定出 3'非翻译区的一个单核苷酸多态性（SNP，c.*2041T>C）。根据对 100 头伯克夏猪的关联分析，该 SNP 与 FA 组成（MUFA 和多不饱和 FA [PUFA]）、多不饱和与饱和 FA 比值（P:S）、n-6:n-3 FA 比值以及肌内脂肪和大理石纹等脂肪沉积程度显著相关（p<0.05）。此外，该 SNP 对 SCD mRNA 表达水平有显著影响（p = 0.041）。基于我们的结果，我们认为 SCD c.*2041T>C SNP 在外显子中起作用，影响了伯克夏猪的肥胖特性。
