Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea.
Mol Biol Rep. 2013 Jan;40(1):73-80. doi: 10.1007/s11033-012-2014-0. Epub 2012 Nov 3.
Fatty acid (FA) composition is one of the most important parameters for the evaluation of meat quality. The stearoyl-CoA desaturase (SCD) gene is considered a positional candidate gene affecting FA composition in SSC14, based on previous quantitative trait loci studies. To evaluate the association of the SCD gene with FA composition in a Korean native pig × Landrace F(2) intercross population, we genotyped six single nucleotide polymorphisms (SNPs) of the SCD gene located in promoter region [2 SNPs (g.-353T>C, g.-233T>C)], exonic region [1 SNP (g.817C>T) in exon 2] and 3' UTR [3 SNPs (g.13311C>G, g.14384G>A, and g.14424C>T)] identified by massively parallel sequencing technology. Eighteen FA composition traits were measured in more than the 950 F(2) animals. A mixed-effect model was used to evaluate associations between these SNPs and FA composition traits in the F(2) intercross population. A detailed investigation detected that the five FA composition traits [palmitoleic acid (C16:1), stearic acid (C18:0), arachidic acid (C20:0), saturated FA, and unsaturated FA] were highly significant (P < 4.7 × 10(-5); C20:0) in association with the SNP g.-233T>C, SNP g.817C>T, SNP g.13311C>G and SNP g.14384G>A in the SCD gene, whereas SNP g.14424C>T was only significantly associated with palmitoleic acid (C16:1, P = 1.4 × 10(-3)). No significant association of FA composition traits with SNP g.-353T>C was detected. In particular, the SNP g.14384G>A accounted for 30.6 % of the additive genetic variance of palmitoleic acid (P = 1.9 × 10(-10)). These results suggest the SCD gene has a strong effect on FA composition in the crossbred pig population.
脂肪酸（FA）组成是评估肉质的最重要参数之一。根据之前的数量性状位点研究，硬脂酰辅酶 A 去饱和酶（SCD）基因被认为是影响 SSC14 中 FA 组成的位置候选基因。为了评估 SCD 基因与韩国本地猪×长白猪 F2 杂交群体中 FA 组成的关联，我们对位于启动子区域[2 个 SNP（g.-353T>C、g.-233T>C）]、外显子区域[1 个 SNP（g.817C>T）第 2 外显子]和 3'UTR [3 个 SNP（g.13311C>G、g.14384G>A 和 g.14424C>T）]的 SCD 基因的 6 个单核苷酸多态性（SNP）进行了基因分型，这些 SNP 是通过大规模平行测序技术鉴定的。在超过 950 个 F2 动物中测量了 18 个 FA 组成性状。使用混合效应模型评估了这些 SNP 与 F2 杂交群体中 FA 组成性状之间的关联。详细调查发现，五个 FA 组成性状[棕榈油酸（C16:1）、硬脂酸（C18:0）、花生酸（C20:0）、饱和 FA 和不饱和 FA]与 SCD 基因中的 SNP g.-233T>C、SNP g.817C>T、SNP g.13311C>G 和 SNP g.14384G>A 高度相关（P < 4.7×10(-5)；C20:0），而 SNP g.14424C>T 仅与棕榈油酸（C16:1，P = 1.4×10(-3))显著相关。未检测到 FA 组成性状与 SNP g.-353T>C 显著相关。特别是，SNP g.14384G>A 占棕榈油酸（P = 1.9×10(-10)) 加性遗传变异的 30.6%。这些结果表明，SCD 基因对杂交猪群体的 FA 组成有很强的影响。
