Cui Jin, Xie Jiexiong, Gao Ming, Zhou Han, Chen Yao, Cui Tiantian, Bai Xiaolei, Wang Heng, Zhang Guihong
College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China.
Antivir Ther. 2015;20(6):565-72. doi: 10.3851/IMP2924. Epub 2015 Jan 5.
Porcine reproductive and respiratory syndrome (PRRS) is one of the most serious diseases affecting the swine industry worldwide; however, there are no efficient control strategies against PRRS virus (PRRSV) at present. Therefore, development of new antiviral treatment strategies is urgently needed. As reported, lithium chloride (LiCl) can efficiently impair the replication of a variety of viruses, including infectious bronchitis coronavirus (IBV) and transmissible gastroenteritis coronavirus (TGEV). In this report, we explored whether LiCl had the potential to inhibit PRRSV infection.
MARC-145 cells were treated with LiCl at various stages of PRRSV life cycle. Virus titration assay was performed to determine the virus infectivity. The expression of viral mRNA and protein was measured by real-time PCR and indirect immunofluorescence assay, respectively. The transcript levels of cytokines were evaluated by real-time PCR.
LiCl significantly suppressed the synthesis of viral RNA and protein; however, it did not block PRRSV binding and entry. Further studies confirmed that LiCl inhibited PRRSV replication at an early stage and TNF-α, an antiviral cytokine, was significantly increased after LiCl treatment. Thus, we suggested that LiCl inhibited PRRSV infection by up-regulating the level of antiviral cytokine TNF-α at an early infection stage.
Our findings imply that the LiCl has the potential to be used as anti-PRRSV therapy.
猪繁殖与呼吸综合征（PRRS）是影响全球养猪业的最严重疾病之一；然而，目前尚无针对猪繁殖与呼吸综合征病毒（PRRSV）的有效控制策略。因此，迫切需要开发新的抗病毒治疗策略。据报道，氯化锂（LiCl）可有效抑制多种病毒的复制，包括传染性支气管炎冠状病毒（IBV）和传染性胃肠炎冠状病毒（TGEV）。在本报告中，我们探究了LiCl是否具有抑制PRRSV感染的潜力。
在PRRSV生命周期的各个阶段用LiCl处理MARC-145细胞。进行病毒滴定试验以确定病毒感染性。分别通过实时PCR和间接免疫荧光试验测量病毒mRNA和蛋白质的表达。通过实时PCR评估细胞因子的转录水平。
LiCl显著抑制病毒RNA和蛋白质的合成；然而，它并未阻断PRRSV的结合和进入。进一步研究证实，LiCl在早期抑制PRRSV复制，且LiCl处理后抗病毒细胞因子TNF-α显著增加。因此，我们认为LiCl在感染早期通过上调抗病毒细胞因子TNF-α的水平来抑制PRRSV感染。
我们的研究结果表明LiCl有潜力用作抗PRRSV治疗药物。
