State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, Gansu Province, China.
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
J Med Virol. 2017 Nov;89(11):2041-2046. doi: 10.1002/jmv.24821. Epub 2017 Aug 28.
Foot-and-mouth disease virus (FMDV) causes an economically important and highly contagious disease of cloven-hoofed animals such as cattle, swine, and sheep. FMD vaccine is the traditional way to protect against the disease, which can greatly reduce its occurrence. However, the use of FMD vaccines to protect early infection is limited. Therefore, the alternative strategy of applying antiviral agents is required to control the spread of FMDV in outbreak situations. As previously reported, LiCl has obviously inhibition effects on a variety of viruses such as transmissible gastroenteritis virus (TGEV), infectious bronchitis coronavirus (IBV), and pseudorabies herpesvirus and EV-A71 virus. In this study, our findings were the first to demonstrate that LiCl inhibition of the FMDV replication. In this study, BHK-21 cell was dose-dependent with LiCl at various stages of FMDV. Virus titration assay was calculated by the 50% tissue culture infected dose (TCID ) with the Reed and Muench method. The cytotoxicity assay of LiCl was performed by the CCK8 kit. The expression level of viral mRNA was measured by RT-qPCR. The results revealed LiCl can inhibit FMDV replication, but it cannot affect FMDV attachment stage and entry stage in the course of FMDV life cycle. Further studies confirmed that the LiCl affect the replication stage of FMDV, especially the early stages of FMDV replication. So LiCl has potential as an effective anti-FMDV drug. Therefore, LiCl may be an effective drug for the control of FMDV. Based on that, the mechanism of the antiviral effect of LiCl on FMDV infection is need to in-depth research in vivo.
口蹄疫病毒（FMDV）会引起偶蹄动物（如牛、猪和羊）的一种具有重要经济意义且高度传染性的疾病。口蹄疫疫苗是预防这种疾病的传统方法，可以大大减少其发生。然而，使用口蹄疫疫苗来预防早期感染是有限的。因此，需要应用抗病毒药物的替代策略来控制口蹄疫病毒在疫情爆发时的传播。如前所述，LiCl 对多种病毒（如传染性胃肠炎病毒（TGEV）、传染性支气管炎冠状病毒（IBV）、伪狂犬病疱疹病毒和 EV-A71 病毒）具有明显的抑制作用。在本研究中，我们的发现首次表明 LiCl 抑制口蹄疫病毒的复制。在本研究中，BHK-21 细胞在口蹄疫病毒的各个阶段都表现出剂量依赖性的 LiCl 抑制作用。病毒滴定试验通过 Reed 和 Muench 法计算 50%组织培养感染剂量（TCID ）。用 CCK8 试剂盒测定 LiCl 的细胞毒性。用 RT-qPCR 测定病毒 mRNA 的表达水平。结果表明，LiCl 能抑制口蹄疫病毒的复制，但不能影响口蹄疫病毒在生命周期中的附着阶段和进入阶段。进一步的研究证实，LiCl 影响口蹄疫病毒的复制阶段，特别是口蹄疫病毒复制的早期阶段。因此，LiCl 可能是一种有效的抗口蹄疫病毒药物。因此，LiCl 可能是控制口蹄疫病毒的有效药物。基于此，需要在体内深入研究 LiCl 对口蹄疫病毒感染的抗病毒作用机制。
