Fekrazad Reza, Ghasemi Barghi Vadood, Poorsattar Bejeh Mir Arash, Shams-Ghahfarokhi Masoumeh
Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran; Department of Periodontics, Dental Faculty, AJA University of Medical Sciences, Tehran, Iran.
Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran; Department of Periodontics, Ardabil University of Medical Sciences, Ardabil, Iran.
Photodiagnosis Photodyn Ther. 2015 Mar;12(1):52-7. doi: 10.1016/j.pdpdt.2014.12.006. Epub 2015 Jan 3.
The application of a new generation of photosensitizers to increase the efficacy of antifungal photodynamic therapy (aPDT) is an important aspect of PDT. Thus, this in vitro study is aimed to evaluate the antifungal efficacy of the photo-elimination of Candida albicans with photothermal and antifungal photodynamic therapy.
aPDT with new methylene blue and photothermal therapy with EmunDo® were applied to a fungal suspension, which was then subcultured in Sabouraud dextrose agar (SDA). The C. albicans colonies were counted and are expressed as colony-forming unit per milliliter (CFU/ml).
aPDT with either EmunDo® or new methylene blue (NMB) considerably diminished the viability of inoculated C. albicans (P<0.001) by log reduction of 1.9 and 3.37, respectively, compared with the control group respectively, compared with the control group. The antifungal potency or dark toxicity of the two photosensitizers alone did not significantly differ (P=0.70). The same trend was observed for the light sources (λ: 810nm vs. λ: 630nm), which also did not significantly differ (P=0.78).
The photo-elimination of C. albicans with either new methylene blue or EmunDo® as a photosensitizer can reduce the viability of fungal cells. Although the result of this study is encouraging, further investigations are warranted to determine clear protocols for the reliable and safe application of this method in clinical practice.
应用新一代光敏剂以提高抗真菌光动力疗法（aPDT）的疗效是光动力疗法的一个重要方面。因此，本体外研究旨在评估采用光热和抗真菌光动力疗法光消除白色念珠菌的抗真菌疗效。
将含新亚甲蓝的aPDT和采用EmunDo®的光热疗法应用于真菌悬液，然后将其接种于沙氏葡萄糖琼脂（SDA）中进行传代培养。对白色念珠菌菌落进行计数，并以每毫升菌落形成单位（CFU/ml）表示。
与对照组相比，采用EmunDo®或新亚甲蓝（NMB）的aPDT分别使接种的白色念珠菌活力显著降低（P<0.001），对数减少分别为1.9和3.37。单独使用两种光敏剂的抗真菌效力或暗毒性无显著差异（P=0.70）。对于光源（波长：810nm与波长：630nm）也观察到相同趋势，且也无显著差异（P=0.78）。
以新亚甲蓝或EmunDo®作为光敏剂光消除白色念珠菌可降低真菌细胞活力。尽管本研究结果令人鼓舞，但仍需进一步研究以确定在临床实践中可靠且安全应用该方法的明确方案。
