Fekrazad Reza, Poorsattar Bejeh Mir Arash, Ghasemi Barghi Vadood, Shams-Ghahfarokhi Masoomeh
Laser research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran; Periodontology Department, Dental Faculty, AJA University of Medical Sciences, Tehran, Iran.
Laser research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran; Dental Materials Research Center, Babol University of Medical Sciences, Babol, Iran.
Photodiagnosis Photodyn Ther. 2015 Jun;12(2):289-97. doi: 10.1016/j.pdpdt.2014.12.009. Epub 2015 Jan 5.
We aimed to evaluate the efficacy of alternative therapies rather than the current antifungal conventional therapy and with assessing the hypothesis of photoactivation of citrus essential oil, fluconazole and Indocyanine green to treat two common mucocutaneous fungal infections.
Suspensions of Candida albicans and Tricophyton rubrum containing 10(6)cells/ml was prepared. Equal samples were treated with infrared (IR) laser irradiation (810 nm, 55 J/cm(2)) in the presence of Indocyanine green (Emundo, 1 mg/ml) (IRLE), photoactivated Citrus aurantifolia essential oil (EO) with sequential exposure to natural and tungsten lights (CE), control non-activated essential oil (CC), laser alone (IRL), indocyanine green alone (E) and neither of treatments as the control group (C). Additional fluconazole (FL, 25.6 μg/ml) and IR activated fluconazole (IRLFL) groups were designed for T. rubrum fungi. Inoculums were serially diluted to 10(-2) and 10(-4) and streaked on Sabouraud dextrose agar plates. Final outcomes were assessed as the percent of reduction.
Cell reduction rates (%) in C. albicans groups were 99.99 (CE), 91.67 (IRLE), 86.67 (CC), 72.37 (E) and 67.27 (RL). Whereas, a 99.99 (CE), 89.99 (CC), 74.5 (IRLE), 64.5 (E), 38.5 (IRLF), 37.5 (RL), and 31 (FL) percent eradication was achieved in T. rubrum groups.
Photoactivation of Citrus EO increased the killing capability by 10-13%. A modest 7.5% augmented effect was observed with IR activation of Fluconazole. Both Citrus EO and photothermal-photodynamic therapy with ICG and IR diode laser exhibited remarkable lethal effect on fungal cells. Candida viable cells are more susceptible to laser only and ICG only treatments than Tricophyton cells.
我们旨在评估替代疗法而非当前抗真菌传统疗法的疗效，并验证柑橘精油、氟康唑和吲哚菁绿光活化治疗两种常见黏膜皮肤真菌感染的假说。
制备含10⁶个细胞/毫升的白色念珠菌和红色毛癣菌悬液。等量样本在吲哚菁绿（Emundo，1毫克/毫升）存在的情况下接受红外（IR）激光照射（810纳米，55焦耳/平方厘米）（IRLE），光活化的香橼叶精油（EO）先后暴露于自然光和钨灯下（CE），对照未活化精油（CC），单独激光照射（IRL），单独吲哚菁绿（E），以及不进行任何处理作为对照组（C）。针对红色毛癣菌还设计了额外的氟康唑（FL，25.6微克/毫升）和红外活化氟康唑（IRLFL）组。接种物连续稀释至10⁻²和10⁻⁴，然后划线接种于沙氏葡萄糖琼脂平板上。最终结果以减少百分比来评估。
白色念珠菌组的细胞减少率（%）分别为99.99（CE）、91.67（IRLE）、86.67（CC）、72.37（E）和67.27（RL）。而在红色毛癣菌组中，根除率分别为99.99（CE）、89.99（CC）、74.5（IRLE）、64.5（E）、38.5（IRLF）、37.5（RL）和31（FL）。
香橼叶精油的光活化使杀伤能力提高了10 - 13%。氟康唑的红外活化观察到适度的7.5%增强效果。香橼叶精油以及吲哚菁绿和红外二极管激光的光热 - 光动力疗法对真菌细胞均表现出显著的致死作用。白色念珠菌活细胞比红色毛癣菌细胞对单独激光照射和单独吲哚菁绿处理更敏感。
