Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, P.O. Box 91775-1365, Mashhad, Iran.
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
Biosens Bioelectron. 2015 Jun 15;68:95-106. doi: 10.1016/j.bios.2014.12.052. Epub 2014 Dec 24.
Up to now, many different methods have been developed for detection of cocaine, but most of these methods are usually time-consuming, tedious and require special or expensive equipment. Therefore, the development of simple, sensitive and rapid detection methods is necessary. In the last decade, aptamers have been used as a new biosensor platform for detection of cocaine in different samples. Aptamers are artificial single-stranded DNA or RNA oligonucleotides capable of binding to specific molecular targets with high affinity and if integrated to nanomaterials, it may lead in precise methods for cocaine detection in the common laboratories. In this review, recent advances and applications of aptamer-based biosensors and nanobiosensors, have been updated, paying attention to the use of fluorescence, colorimetric and electrochemical techniques for the detection and quantitative determination of cocaine.
到目前为止，已经开发出许多不同的可卡因检测方法，但这些方法大多通常耗时、繁琐，需要特殊或昂贵的设备。因此，开发简单、灵敏和快速的检测方法是必要的。在过去的十年中，适体已被用作一种新的生物传感器平台，用于检测不同样本中的可卡因。适体是能够与特定分子靶标高亲和力结合的人工单链 DNA 或 RNA 寡核苷酸，如果与纳米材料结合，可能会导致在普通实验室中检测可卡因的精确方法。本综述更新了基于适体的生物传感器和纳米生物传感器的最新进展和应用，重点介绍了荧光、比色和电化学技术在可卡因检测和定量测定中的应用。
