Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Biosens Bioelectron. 2018 Jul 15;111:1-9. doi: 10.1016/j.bios.2018.03.070. Epub 2018 Apr 3.
Utilization of traditional analytical techniques is limited because they are generally time-consuming and require high consumption of reagents, complicated sample preparation and expensive equipment. Therefore, it is of great interest to achieve sensitive, rapid and simple detection methods. It is believed that nucleic acids assays, especially aptamers, are very important in modern life sciences for target detection and biological analysis. Aptamers and DNA-based sensors have been widely used for the design of various sensors owing to their unique features. In recent years, triple-helix molecular switch (THMS)-based aptasensors and DNA sensors have been broadly utilized for the detection and analysis of different targets. The THMS relies on the formation of DNA triplex via Watson-Crick and Hoogsteen base pairings under optimal conditions. This review focuses on recent progresses in the development and applications of electrochemical, colorimetric, fluorescence and SERS aptasensors and DNA sensors, which are based on THMS. Also, the advantages and drawbacks of these methods are discussed.
传统分析技术的应用受到限制，因为它们通常耗时且需要大量试剂消耗、复杂的样品制备和昂贵的设备。因此，实现敏感、快速和简单的检测方法非常重要。人们相信，核酸分析，特别是适体，在现代生命科学中对于目标检测和生物分析非常重要。适体和基于 DNA 的传感器由于其独特的特性而被广泛用于各种传感器的设计。近年来，基于三螺旋分子开关 (THMS) 的适体传感器和 DNA 传感器已被广泛用于不同目标的检测和分析。THMS 依赖于在最佳条件下通过 Watson-Crick 和 Hoogsteen 碱基配对形成 DNA 三链体。本综述重点介绍了基于 THMS 的电化学、比色法、荧光和 SERS 适体传感器和 DNA 传感器的最新进展及其应用。此外，还讨论了这些方法的优缺点。
