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枯草芽孢杆菌一个在其他生物体中尚未鉴定出同源物的细胞分裂起始基因的克隆与表达。

Cloning and expression of a Bacillus subtilis division initiation gene for which a homolog has not been identified in another organism.

作者信息

Harry E J, Wake R G

机构信息

Department of Biochemistry, University of Sydney, New South Wales, Australia.

出版信息

J Bacteriol. 1989 Dec;171(12):6835-9. doi: 10.1128/jb.171.12.6835-6839.1989.

DOI:10.1128/jb.171.12.6835-6839.1989
PMID:2556376
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC210583/
Abstract

The Bacillus subtilis 168 division initiation genes defined by the temperature-sensitive mutations ts-1 and ts-12 were cloned into a 10.5-kilobase EcoRI fragment of DNA in the lambda EMBL4 vector. The two genes were separated by approximately 3 kilobases. The gene in which the ts-1 mutation resides was shown to be the same as the B. subtilis homolog of the Escherichia coli ftsZ gene. The other gene was named divIB. It showed no homology to any previously identified gene and coded for a protein of 30.1 kilodaltons which was probably membrane bound.

摘要

由温度敏感突变体ts - 1和ts - 12定义的枯草芽孢杆菌168分裂起始基因被克隆到λEMBL4载体的一段10.5千碱基的DNA EcoRI片段中。这两个基因相隔约3千碱基。ts - 1突变所在的基因被证明与大肠杆菌ftsZ基因的枯草芽孢杆菌同源物相同。另一个基因被命名为divIB。它与任何先前鉴定的基因都没有同源性，编码一个30.1千道尔顿的可能与膜结合的蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2897/210583/74c07caaeb9a/jbacter00178-0454-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2897/210583/74c07caaeb9a/jbacter00178-0454-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2897/210583/74c07caaeb9a/jbacter00178-0454-a.jpg

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