脐带血采集的最佳方法：埃及一家公共脐带血库的试验
Optimal method for collection of umbilical cord blood: an Egyptian trial for a public cord blood bank.
作者信息
Bassiouny M R, El-Chennawi F, Mansour A K, Yahia S, Darwish A
机构信息
Pediatric Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
出版信息
Transfusion. 2015 Jun;55(6):1263-8. doi: 10.1111/trf.12978. Epub 2015 Jan 7.
BACKGROUND
Umbilical cord blood (UCB) contains stem cells and can be used as an alternative to bone marrow transplantation. Engraftment is dependent on the total nucleated cell (TNC) and CD34+ cell counts of the cord blood units. This study was designed to evaluate the effect of the method of collection of the UCB on the yield of the cord blood units.
STUDY DESIGN AND METHODS
Informed consent was obtained from 100 eligible mothers for donation of cord blood. Both in utero and ex utero methods were used for collection. The cord blood volume was measured. The TNC and the CD34+ cell counts were enumerated.
RESULTS
We have found that in utero collection gave significantly larger volumes of cord blood and higher TNC counts than ex utero collection. There was no significant difference between both methods regarding the CD34+ cell counts. This study revealed a significant correlation between the volume of the collected cord blood and both TNC and CD34+ cell counts.
CONCLUSION
It is better to collect cord blood in utero before placental delivery to optimize the quality of the cord blood unit.
背景
脐带血（UCB）含有干细胞，可作为骨髓移植的替代方法。植入取决于脐带血单位的总核细胞（TNC）和CD34+细胞计数。本研究旨在评估脐带血采集方法对脐带血单位产量的影响。
研究设计与方法
获得100名符合条件的母亲的知情同意以捐赠脐带血。采用宫内和宫外两种采集方法。测量脐带血体积。计数TNC和CD34+细胞数量。
结果
我们发现，宫内采集比宫外采集能获得显著更多体积的脐带血和更高的TNC计数。两种方法在CD34+细胞计数方面无显著差异。本研究揭示了采集的脐带血体积与TNC和CD34+细胞计数之间存在显著相关性。
结论
在胎盘娩出前进行宫内脐带血采集以优化脐带血单位质量更佳。
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