Lee Chang-Moon, Lee Tai Kyoung, Kim Dae-Ik, Kim Yu-Ri, Kim Meyoung-Kon, Jeong Hwan-Jeong, Sohn Myung-Hee, Lim Seok Tae
Department of Biomedical Engineering, Chonnam National University, Yeosu, Jeollanam-Do, Republic of Korea.
Department of Nuclear Medicine, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea ; Cyclotron Research Center, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea ; Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea ; Molecular Imaging and Therapeutic Medicine Research Center, Chonbuk National University Medical School and Hospital, Jeonju, Jeollabuk-Do, Republic of Korea.
Int J Nanomedicine. 2014 Dec 15;9 Suppl 2(Suppl 2):243-50. doi: 10.2147/IJN.S57938. eCollection 2014.
In this study, we investigated the absorption and distribution of rhodamine B isothiocyanate (RITC)-incorporated silica oxide nanoparticles(SiNPs) (RITC-SiNPs) after oral exposure, by conducting optical imaging, with a focus on tracking the movement of RITC-SiNPs of different particle size and surface charge.
RITC-SiNPs (20 or 100 nm; positively or negatively charged) were used to avoid the dissociation of a fluorescent dye from nanoparticles via spontaneous or enzyme-catalyzed reactions in vivo. The changes in the nanoparticle sizes and shapes were investigated in an HCl solution for 6 hours. RITC-SiNPs were orally administered to healthy nude mice at a dose of 100 mg/kg. Optical imaging studies were performed at 2, 4, and 6 hours after oral administration. The mice were sacrificed at 2, 4, 6, and 10 hours post-administration, and ex vivo imaging studies were performed.
The RITC-SiNPs were stable in the HCl solution for 6 hours, without dissociation of RITC from the nanoparticles and without changes in size and shape. RITC-SiNPs flowed into the small intestine from the stomach and gradually moved along the gut during the experiment. In the ex vivo imaging studies, optical signals were observed mostly in the lungs, liver, pancreas, and kidneys. The orally administered RITC-SiNPs, which were absorbed in the systemic circulation, were eliminated from the body into the urine. The 20 nm RITC-SiNPs showed higher uptake in the lungs than the 100 nm RITC-SiNPs. The distribution of the 100 nm RITC-SiNPs in the liver was higher than that of the 20 nm RITC-SiNPs, but the differences in the surface charge behavior were imperceptible.
We demonstrated that the movement of RITC-SiNPs after oral exposure could be traced by optical imaging. Optical imaging has the potential to provide valuable information that will help in understanding the behavior of SiNPs in the body following exposure.
在本研究中，我们通过光学成像研究了口服暴露后异硫氰酸罗丹明B（RITC）标记的二氧化硅纳米颗粒（SiNPs）（RITC-SiNPs）的吸收和分布情况，重点是追踪不同粒径和表面电荷的RITC-SiNPs的运动。
使用RITC-SiNPs（20或100纳米；带正电荷或负电荷）以避免荧光染料在体内通过自发或酶催化反应从纳米颗粒上解离。在HCl溶液中研究纳米颗粒尺寸和形状的变化6小时。将RITC-SiNPs以100mg/kg的剂量口服给予健康裸鼠。在口服给药后2、4和6小时进行光学成像研究。在给药后2、4、6和10小时处死小鼠，并进行离体成像研究。
RITC-SiNPs在HCl溶液中6小时内稳定，RITC未从纳米颗粒上解离，尺寸和形状也未改变。在实验过程中，RITC-SiNPs从胃流入小肠并逐渐沿肠道移动。在离体成像研究中，光学信号主要在肺、肝、胰腺和肾脏中观察到。口服给药的RITC-SiNPs被全身循环吸收后，通过尿液排出体外。20纳米的RITC-SiNPs在肺中的摄取量高于100纳米的RITC-SiNPs。100纳米的RITC-SiNPs在肝脏中的分布高于20纳米的RITC-SiNPs，但表面电荷行为的差异不明显。
我们证明了口服暴露后RITC-SiNPs的运动可以通过光学成像追踪。光学成像有潜力提供有价值的信息，有助于理解暴露后SiNPs在体内的行为。
