Wu X Z, Zhang T T, Guo J G, Liu Z, Yang F H, Gao X H
Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun City, Jilin 130112, China.
Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun City, Jilin 130112, China.
J Anim Sci. 2015 Jan;93(1):176-84. doi: 10.2527/jas.2014-8026.
A 3 × 3 + 1 factorial experiment was conducted based on a completely randomized design to evaluate the effects of different sources of copper on plasma metabolites, nutrient digestibility, relative copper bioavailability, and retention of some minerals in male mink. Animals in the control group were fed a basal diet, which mainly consisted of corn, fish meal, meat and bone meal, and soybean oil, with no copper supplementation. Mink in the other 9 treatments were fed the basal diet supplemented with Cu from reagent-grade copper sulfate (CuSO4), tribasic copper chloride (TBCC), or copper methionine (CuMet). Copper concentrations of the experimental diets were 50, 100, and 150 mg Cu/kg DM. Blood samples were collected via the toe clip at the end of study (d 42) to determine blood hematology and blood metabolites. A metabolism trial of 4 d was conducted during the last week of experimental feeding. There was a linear (P < 0.01) effect of dose of Cu on plasma Cu concentrations, ceruloplasmin concentration, and Cu-Zn superoxide dismutase activity. A linear response to Cu dose was noted for fat (P < 0.05) digestibility. Supplemental dose of Cu linearly increased (P < 0.05) liver Cu and decreased (P < 0.05) liver Zn level but did not alter liver Fe. The concentration of liver Cu of the mink fed with TBCC and CuMet diets was greater (P < 0.05) than that fed CuSO4. Compared with CuSO4 (100%), relative bioavailability values of TBCC were 104 and 104%, based on serum ceruloplasmin and liver copper, respectively, and relative bioavailability values of CuMet were 130 and 111%. CuMet and TBCC are more bioavailable than CuSO4. In conclusion, the relative bioavailability of CuMet obtained in this study was greater than that of CuSO4 and TBCC. Dose of Cu had an important effect on the regulating ceruloplasmin concentration, Cu-Zn superoxide dismutase activity, and the digestion of dietary fat in mink.
基于完全随机设计进行了一项3×3 + 1析因试验，以评估不同铜源对雄性水貂血浆代谢物、养分消化率、相对铜生物利用率以及某些矿物质留存率的影响。对照组动物饲喂基础日粮，该日粮主要由玉米、鱼粉、肉骨粉和大豆油组成，未添加铜。其他9个处理组的水貂饲喂添加了试剂级硫酸铜（CuSO4）、碱式氯化铜（TBCC）或蛋氨酸铜（CuMet）的基础日粮。试验日粮的铜浓度分别为50、100和150 mg Cu/kg干物质。在研究结束时（第42天）通过剪趾采集血样，以测定血液学指标和血液代谢物。在试验饲喂的最后一周进行了为期4天的代谢试验。铜剂量对血浆铜浓度、铜蓝蛋白浓度和铜锌超氧化物歧化酶活性有线性（P < 0.01）影响。脂肪消化率（P < 0.05）对铜剂量呈线性反应。补充铜剂量使肝脏铜含量线性增加（P < 0.05），肝脏锌含量降低（P < 0.05），但未改变肝脏铁含量。饲喂TBCC和CuMet日粮的水貂肝脏铜浓度高于（P < 0.05）饲喂CuSO4日粮的水貂。基于血清铜蓝蛋白和肝脏铜，TBCC的相对生物利用率值分别为104%和104%，相对于CuSO4（100%），CuMet的相对生物利用率值分别为130%和111%。CuMet和TBCC的生物利用率高于CuSO4。总之，本研究中获得的CuMet的相对生物利用率高于CuSO4和TBCC。铜剂量对水貂铜蓝蛋白浓度、铜锌超氧化物歧化酶活性和日粮脂肪消化有重要影响。
