Kacinski B M, Carter D, Kohorn E I, Mittal K, Bloodgood R S, Donahue J, Kramer C A, Fischer D, Edwards R, Chambers S K
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06510.
Yale J Biol Med. 1989 Jul-Aug;62(4):379-92.
Six-micron paraffin sections of paraformaldehyde-fixed specimens of 24 ovarian benign and neoplastic specimens were assayed for tumor cell-specific oncogene expression by a sensitive, quantitative in situ hybridization technique with probes for 17 oncogenes, beta-actin, and E. coli beta-lactamase. In the benign, borderline, and invasive adenocarcinomas, multiple oncogenes, including neu, fes, fms, Ha-ras, trk, c-myc, fos, and PDGF-A chains, were expressed at significant levels relative to a housekeeping gene (beta-actin). In the mixed-Mullerian tumors, a rather different pattern of oncogene expression was observed, characterized primarily by expression of sis (PDGF-B chain). For the adenocarcinomas, statistical analysis demonstrated that expression of several genes (fms, neu, PDGF-A) was closely linked to others (c-fos, c-myc) known to have important roles in the control of cell proliferation, but only one gene, fms, correlated very strongly with clinicopathologic features (high FIGO histologic grade and high FIGO clinical stage) predictive of aggressive clinical behavior and poor outcome. The authors discuss the role that tumor epithelial cell expression of the fms gene product might play in the auto- and paracrine control of growth and dissemination of ovarian adenocarcinomas.
采用灵敏的定量原位杂交技术，使用针对17种癌基因、β-肌动蛋白和大肠杆菌β-内酰胺酶的探针，对24例卵巢良性和肿瘤标本的多聚甲醛固定标本的6微米石蜡切片进行肿瘤细胞特异性癌基因表达检测。在良性、交界性和浸润性腺癌中，相对于管家基因（β-肌动蛋白），包括neu、fes、fms、Ha-ras、trk、c-myc、fos和血小板衍生生长因子-A链（PDGF-A链）在内的多种癌基因均有显著表达。在混合性苗勒管肿瘤中，观察到一种截然不同的癌基因表达模式，主要特征为sis（血小板衍生生长因子-B链，PDGF-B链）的表达。对于腺癌，统计分析表明，几种基因（fms、neu、PDGF-A）的表达与已知在细胞增殖控制中起重要作用的其他基因（c-fos, c-myc）密切相关，但只有一个基因fms与预测侵袭性临床行为和不良预后的临床病理特征（高国际妇产科联盟组织学分级和高国际妇产科联盟临床分期）密切相关。作者讨论了fms基因产物在肿瘤上皮细胞中的表达可能在卵巢腺癌生长和扩散的自分泌和旁分泌控制中所起的作用。
