Dorand R Dixon, Barkauskas Deborah S, Evans Teresa A, Petrosiute Agne, Huang Alex Y
Department of Pathology; Case Western Reserve University School of Medicine; Cleveland, Ohio USA.
Department of Biomedical Engineering; Case Western Reserve University School of Medicine; Cleveland, Ohio USA.
Intravital. 2014 May;3(2). doi: 10.4161/intv.29728.
Fluorescent imaging coupled with high-resolution femto-second pulsed infrared lasers allows for interrogation of cellular interactions deeper in living tissues than ever imagined. Intra-vital imaging of the central nervous system (CNS) has provided insights into neuronal development, synaptic transmission, and even immune interactions. In this review we will discuss the two most common intravital approaches for studying the cerebral cortex in the live mouse brain for pre-clinical studies, the thinned skull and cranial window techniques, and focus on the advantages and drawbacks of each approach. In addition, we will discuss the use of neuronal physiologic parameters as determinants of successful surgical and imaging preparation.
荧光成像与高分辨率飞秒脉冲红外激光相结合，能够对活组织中细胞相互作用进行比以往想象更深层次的探究。中枢神经系统（CNS）的活体成像为神经元发育、突触传递甚至免疫相互作用提供了深入见解。在本综述中，我们将讨论用于临床前研究的、在活体小鼠大脑中研究大脑皮层的两种最常见的活体方法，即薄颅骨和颅骨视窗技术，并重点关注每种方法的优缺点。此外，我们将讨论使用神经元生理参数作为成功进行手术和成像准备的决定因素。
