Nakchum Ladawan, Kim Sang Moo
a Department of Marine Food Science and Technology , Gangneung-Wonju National University , Gangneung , South Korea.
Prep Biochem Biotechnol. 2016;46(2):123-30. doi: 10.1080/10826068.2014.995808.
A collagen was isolated from squid skin, a processing waste product. The biofunctional activities of enzymatic squid skin collagen hydrolysates were determined to produce a value-added material. Five low-molecular-mass hydrolysate fractions, F1 (>30 kD), F2 (10-30 kD), F3 (3-10 kD), F4 (1-3 kD), and F5 (<1 kD), were manufactured from its enzymatic hydrolysate by ultrafiltration. Fraction F3 had the strongest antihyaluronidase inhibitory activity. Gly, Val, and Pro were major amino acids in F3, while Met, Tyr, and His were minor ones. The molecular mass of F3 was in the range of 3.4 to 10 kD. F3 exhibited copper chelating ability in a concentration-dependent manner. The ferrous chelating ability of F3 was almost 50% at 200 µg/mL. F3 also inhibited tyrosinase activity by 39.65% at 1 mg/mL. Furthermore, F3 had stronger hydroxyl radical scavenging activity (IC50 = 149.94 µg/mL) than ascorbic acid (IC50 = 212.94 µg/mL). Therefore, the squid collagen hydrolysate can be utilized as a nutraceutical or cosmeceutical agent.
从鱿鱼皮（一种加工废料）中分离出一种胶原蛋白。测定了酶解鱿鱼皮胶原蛋白水解产物的生物功能活性，以生产一种增值材料。通过超滤从其酶解产物中制备了五个低分子量水解产物级分，即F1（>30 kD）、F2（10 - 30 kD）、F3（3 - 10 kD）、F4（1 - 3 kD）和F5（<1 kD）。F3级分具有最强的抗透明质酸酶抑制活性。甘氨酸、缬氨酸和脯氨酸是F3中的主要氨基酸，而甲硫氨酸、酪氨酸和组氨酸是次要氨基酸。F3的分子量在3.4至10 kD范围内。F3以浓度依赖性方式表现出铜螯合能力。在200 µg/mL时，F3的亚铁螯合能力约为50%。F3在1 mg/mL时还能抑制酪氨酸酶活性3
