Monzo Mariano, Martínez-Rodenas Francisco, Moreno Isabel, Navarro Alfons, Santasusagna Sandra, Macias Ismael, Muñoz Carmen, Tejero Rut, Hernández Raquel
From the Molecular Oncology and Embryology Laboratory, Human Anatomy Unit, School of Medicine, University of Barcelona, IDIBAPS, Barcelona (MM, AN, SS, IM, CM, RT); Department of Medical Oncology and Surgery, Hospital Municipal de Badalona, Badalona, Spain (FM-R, IM, RH).
Medicine (Baltimore). 2015 Jan;94(1):e145. doi: 10.1097/MD.0000000000000145.
Findings on the role of plasma miR-21 expression in colorectal cancer are contradictory. Before reaching a peripheral vein (PV), microRNAs released by the tumor are dispersed throughout the body. We hypothesized that blood drawn from the mesenteric vein (MV) near the site of the primary tumor could provide more homogeneous information than blood drawn from the PV.We have analyzed miR-21 expression in matched samples of tumor tissue, normal tissue, MV plasma, and PV plasma in 57 surgically resected patients with colon cancer and correlated our findings with clinical characteristics and disease-free survival (DFS).miR-21 expression was higher in MV than PV plasma (P = 0.014) and in tumor than in normal tissue (P < 0.001). Patients with high levels of miR-21 in MV plasma had shorter DFS (P = 0.05) than those with low levels, and those with high levels in both MV and PV plasma had shorter DFS than all other patients (P = 0.01).Our findings suggest that the primary tumor in colon cancer releases high concentrations of miR-21 in the MV but that these concentrations are later diluted in the circulatory system. MV expression of miR-21 may be a stronger prognostic marker than PV expression.
关于血浆miR - 21表达在结直肠癌中的作用，研究结果相互矛盾。在进入外周静脉（PV）之前，肿瘤释放的微小RNA会散布于全身。我们推测，从原发性肿瘤部位附近的肠系膜静脉（MV）抽取的血液可能比从PV抽取的血液提供更一致的信息。我们分析了57例接受手术切除的结肠癌患者肿瘤组织、正常组织、MV血浆和PV血浆的配对样本中miR - 21的表达情况，并将我们的研究结果与临床特征和无病生存期（DFS）相关联。miR - 21在MV中的表达高于PV血浆（P = 0.014），在肿瘤组织中的表达高于正常组织（P < 0.001）。MV血浆中miR - 21水平高的患者DFS短于水平低的患者（P = 0.05），MV和PV血浆中miR - 21水平均高的患者DFS短于所有其他患者（P = 0.01）。我们的研究结果表明，结肠癌的原发性肿瘤在MV中释放高浓度的miR - 21，但这些浓度随后在循环系统中被稀释。miR - 21在MV中的表达可能比在PV中的表达是更强的预后标志物。
