Yanagi K, Harada S
Department of Virology and Rickettsiology, National Institute of Health, Tokyo, Japan.
Arch Virol. 1989;108(1-2):151-9. doi: 10.1007/BF01313753.
The infectivity of herpes simplex virus type 1 (HSV-1) was found to be markedly reduced by treating virions with the tertiary amine local anesthetics lidocaine, dibucaine and tetracaine. These treatments induced a characteristic shift in the buoyant density of the HSV-1 particles from a "light" to a "heavy" population. HSV-1 virions were unstable at alkaline pH, and alkali treatment caused the same shift in buoyant density. Ca2+ stabilized the "light" population. These results suggest that the physicochemical status of the HSV-1 envelope which is sensitive to the treatments described above plays an important role for the integrity of the virion.
研究发现，用叔胺类局部麻醉药利多卡因、丁卡因和丁哌卡因处理单纯疱疹病毒1型（HSV-1）病毒粒子后，其感染性显著降低。这些处理导致HSV-1颗粒的浮力密度发生特征性变化，从“轻”群体转变为“重”群体。HSV-1病毒粒子在碱性pH下不稳定，碱处理也导致浮力密度发生相同变化。Ca2+可稳定“轻”群体。这些结果表明，HSV-1包膜的物理化学状态对上述处理敏感，对病毒粒子的完整性起着重要作用。
