使用单个480nm宽带光源对视网膜进行同步光学相干断层扫描和脂褐素自发荧光成像。
Simultaneous optical coherence tomography and lipofuscin autofluorescence imaging of the retina with a single broadband light source at 480nm.
作者信息
Jiang Minshan, Liu Tan, Liu Xiaojing, Jiao Shuliang
机构信息
Engineering Research Center of Optical Instruments and Systems, Ministry of Education, Shanghai Key Lab of Modern Optical Systems, University of Shanghai for Science and Technology, No. 516 Jungong Road, Shanghai 200093, China ; Department of Biomedical Engineering, Florida International University, 10555 W Flagler Street, Miami, Florida, 33174, USA.
Department of Biomedical Engineering, Florida International University, 10555 W Flagler Street, Miami, Florida, 33174, USA.
出版信息
Biomed Opt Express. 2014 Nov 12;5(12):4242-8. doi: 10.1364/BOE.5.004242. eCollection 2014 Dec 1.
We accomplished spectral domain optical coherence tomography and auto-fluorescence microscopy for imaging the retina with a single broadband light source centered at 480 nm. This technique is able to provide simultaneous structural imaging and lipofuscin molecular contrast of the retina. Since the two imaging modalities are provided by the same group of photons, their images are intrinsically registered. To test the capabilities of the technique we periodically imaged the retinas of the same rats for four weeks. The images successfully demonstrated lipofuscin accumulation in the retinal pigment epithelium with aging. The experimental results showed that the dual-modal imaging system can be a potentially powerful tool in the study of age-related degenerative retinal diseases.
我们利用一个中心波长为480nm的单宽带光源完成了光谱域光学相干断层扫描和自体荧光显微镜检查，用于视网膜成像。该技术能够同时提供视网膜的结构成像和脂褐素分子对比度。由于这两种成像方式由同一组光子提供，它们的图像在本质上是配准的。为了测试该技术的能力，我们在四周内定期对同一只大鼠的视网膜进行成像。图像成功地显示了随着年龄增长视网膜色素上皮中脂褐素的积累。实验结果表明，这种双模态成像系统可能成为研究与年龄相关的视网膜退行性疾病的有力工具。
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