基于可见光光学相干断层扫描技术并使用标准参考靶标的视网膜色素上皮中脂褐素的定量成像。
Visible light OCT-based quantitative imaging of lipofuscin in the retinal pigment epithelium with standard reference targets.
作者信息
Nafar Zahra, Wen Rong, Jiao Shuliang
机构信息
Department of Biomedical Engineering, Florida International University, 10555 W Flagler St, Miami, FL 33174, USA.
Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10 Ave, Miami, FL 33136, USA.
出版信息
Biomed Opt Express. 2018 Jul 23;9(8):3768-3782. doi: 10.1364/BOE.9.003768. eCollection 2018 Aug 1.
Abstract
We developed a technology for quantitative retinal autofluorescence (AF, or FAF for fundus AF) imaging for quantifying lipofuscin in the retinal pigment epithelium (RPE). The technology is based on simultaneous visible light optical coherence tomography (VIS-OCT) and AF imaging of the retina and a pair of reference standard targets at the intermediate retinal imaging plane with known reflectivity for the OCT and fluorescence efficiency for the FAF. The technology is able to eliminate the pre-RPE attenuation in FAF imaging by using the simultaneously acquired VIS-OCT image. With the OCT and fluorescence images of the reference targets, the effects of illumination power and detector sensitivity can be eliminated.
摘要
我们开发了一种用于定量视网膜自发荧光（AF，或眼底AF的FAF）成像的技术，用于定量视网膜色素上皮（RPE）中的脂褐素。该技术基于同步可见光光学相干断层扫描（VIS-OCT）以及视网膜和一对位于视网膜中间成像平面的参考标准目标的AF成像，这些参考标准目标对于OCT具有已知反射率，对于FAF具有已知荧光效率。该技术能够通过使用同时采集的VIS-OCT图像消除FAF成像中RPE前的衰减。利用参考目标的OCT和荧光图像，可以消除照明功率和探测器灵敏度的影响。
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