利用光声眼底镜和自发荧光成像技术对视网膜中的黑色素和脂褐素进行体内同时成像。
Simultaneous in vivo imaging of melanin and lipofuscin in the retina with photoacoustic ophthalmoscopy and autofluorescence imaging.
出版信息
J Biomed Opt. 2011 Aug;16(8):080504. doi: 10.1117/1.3606569.
Abstract
We combined photoacoustic ophthalmoscopy (PAOM) with autofluorescence imaging for simultaneous in vivo imaging of dual molecular contrasts in the retina using a single light source. The dual molecular contrasts come from melanin and lipofuscin in the retinal pigment epithelium (RPE). Melanin and lipofuscin are two types of pigments and are believed to play opposite roles (protective versus exacerbate) in the RPE in the aging process. We have successfully imaged the retina of pigmented and albino rats at different ages. The experimental results showed that multimodal PAOM system can be a potentially powerful tool in the study of age-related degenerative retinal diseases.
摘要
我们将光声眼科学（PAOM）与自发荧光成像相结合，使用单个光源同时对视网膜中的两种分子对比进行体内成像。这两种分子对比来自视网膜色素上皮（RPE）中的黑色素和脂褐素。黑色素和脂褐素是两种类型的色素，据信在衰老过程中在 RPE 中发挥相反的作用（保护与加剧）。我们已经成功地对不同年龄的色素沉着和白化大鼠的视网膜进行了成像。实验结果表明，多模态 PAOM 系统可能成为研究与年龄相关的退行性视网膜疾病的有力工具。
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本文引用的文献
1
Simultaneous dual molecular contrasts provided by the absorbed photons in photoacoustic microscopy.
Opt Lett. 2010 Dec 1;35(23):4018-20. doi: 10.1364/OL.35.004018.
2
Photoacoustic ophthalmoscopy for in vivo retinal imaging.
Opt Express. 2010 Feb 15;18(4):3967-72. doi: 10.1364/OE.18.003967.
3
Simultaneous multimodal imaging with integrated photoacoustic microscopy and optical coherence tomography.
Opt Lett. 2009 Oct 1;34(19):2961-3. doi: 10.1364/OL.34.002961.
4
Monitoring the accumulation of lipofuscin in aging murine eyes by fluorescence spectroscopy.
Photochem Photobiol. 2009 Jan-Feb;85(1):234-8. doi: 10.1111/j.1751-1097.2008.00425.x. Epub 2008 Aug 27.
5
Loss of melanin from human RPE with aging: possible role of melanin photooxidation.
Exp Eye Res. 2003 Jan;76(1):89-98. doi: 10.1016/s0014-4835(02)00247-6.
6
Spectral profiling of autofluorescence associated with lipofuscin, Bruch's Membrane, and sub-RPE deposits in normal and AMD eyes.
Invest Ophthalmol Vis Sci. 2002 Jul;43(7):2435-41.
7
Age-related accumulation and spatial distribution of lipofuscin in RPE of normal subjects.
Invest Ophthalmol Vis Sci. 2001 Jul;42(8):1855-66.
8
In vivo fluorescence of the ocular fundus exhibits retinal pigment epithelium lipofuscin characteristics.
Invest Ophthalmol Vis Sci. 1995 Mar;36(3):718-29.
9
Retinal pigment epithelial lipofuscin and melanin and choroidal melanin in human eyes.
Invest Ophthalmol Vis Sci. 1986 Feb;27(2):145-52.
10
An antioxidative role of ocular screening pigments.
Vision Res. 1987;27(6):893-9. doi: 10.1016/0042-6989(87)90005-8.
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