人感染 H10N8 禽流感病毒偏好禽类受体结合的结构基础。

Structural basis for preferential avian receptor binding by the human-infecting H10N8 avian influenza virus.

机构信息

1] College of Veterinary Medicine, China Agricultural University, Beijing 100193, China [2] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

Nat Commun. 2015 Jan 9;6:5600. doi: 10.1038/ncomms6600.

DOI:10.1038/ncomms6600

PMID:25574798

Abstract

Since December 2013, at least three cases of human infections with H10N8 avian influenza virus have been reported in China, two of them being fatal. To investigate the epidemic potential of H10N8 viruses, we examined the receptor binding property of the first human isolate, A/Jiangxi-Donghu/346/2013 (JD-H10N8), and determined the structures of its haemagglutinin (HA) in complex with both avian and human receptor analogues. Our results suggest that JD-H10N8 preferentially binds the avian receptor and that residue R137-localized within the receptor-binding site of HA-plays a key role in this preferential binding. Compared with the H7N9 avian influenza viruses, JD-H10N8 did not exhibit the enhanced binding to human receptors observed with the prevalent H7N9 virus isolate Anhui-1, but resembled the receptor binding activity of the early-outbreak H7N9 isolate (Shanghai-1). We conclude that the H10N8 virus is a typical avian influenza virus.

摘要

自 2013 年 12 月以来，中国至少报告了三例人感染 H10N8 禽流感病毒的病例，其中两例是致命的。为了研究 H10N8 病毒的流行潜力，我们检测了首例人感染 H10N8 病毒的受体结合特性，并确定了其血凝素（HA）与人及禽类受体类似物复合物的结构。结果表明，JD-H10N8 优先结合禽类受体，HA 受体结合部位的 R137 残基在这种优先结合中起关键作用。与 H7N9 禽流感病毒相比，JD-H10N8 并未表现出与流行的 H7N9 病毒分离株 Anhui-1 相同的对人受体的增强结合，但与早期暴发的 H7N9 病毒分离株（Shanghai-1）的受体结合活性相似。我们的结论是，H10N8 病毒是一种典型的禽流感病毒。