Guzelmeric Etil, Vovk Irena, Yesilada Erdem
Yeditepe University, Faculty of Pharmacy, Department of Pharmacognosy and Phytotherapy, Kayisdagi Cad., Atasehir, 34755 Istanbul, Turkey.
National Institute of Chemistry, Laboratory for Food Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia; EN-FIST Centre of Excellence, Trg Osvobodilne fronte 13, SI-1000 Ljubljana, Slovenia.
J Pharm Biomed Anal. 2015 Mar 25;107:108-18. doi: 10.1016/j.jpba.2014.12.021. Epub 2014 Dec 23.
Brewed tea of chamomile flowers (Matricaria recutita L.) (Asteraceae) has been extensively consumed for centuries due to either its pleasant taste or medicinal purposes. On the other hand, the major problem is difficulty in distinguishing the genuine specimen when supplying chamomile through nature-picking. Consequently flowers of other Asteraceae members resembling to chamomile in appearance may frequently be practiced by lay people or marketed in spice shops or bazaars. Evidently detection of such adulterations plays a vital role in terms of public health to avoid risk of toxicity (i.e. pyrazolidin alkaloids) and ineffective treatments (lack or insufficient concentration of the active constituents). This work presents either development and validation of a high performance thin-layer chromatographic (HPTLC) method for apigenin 7-O-glucoside which is one of the active markers in chamomile flowers or its application for the fingerprint discrimination of chamomile-like materials i.e. Anthemis spp., Bellis spp., Chrysanthemum sp. and Tanacetum sp. gathered by local people assuming as chamomile. Separation was performed on the silica gel 60 NH2 F254s HPTLC plates using the developing solvent system of ethyl acetate-formic acid-acetic acid-water (30:1.5:1.5:3, v/v/v/v). The proposed HPTLC method may also be a leading guide for the quality assessment of chamomile tea products on the market.
洋甘菊花（母菊L.）（菊科）泡制的茶因其宜人的口感或药用价值，几个世纪以来一直被广泛饮用。另一方面，主要问题是通过自然采摘供应洋甘菊时，难以辨别真品。因此，外观与洋甘菊相似的其他菊科植物的花朵可能经常被外行人士使用，或在香料店或集市上销售。显然，检测此类掺假对于公共卫生至关重要，以避免毒性风险（即吡唑烷生物碱）和无效治疗（活性成分缺乏或浓度不足）。这项工作介绍了洋甘菊花中活性标志物之一芹菜素7-O-葡萄糖苷的高效薄层色谱（HPTLC）方法的开发与验证，或其在指纹鉴别类似洋甘菊材料（即被当地人当作洋甘菊采集的春黄菊属、雏菊属、菊属和菊蒿属植物）中的应用。使用乙酸乙酯-甲酸-乙酸-水（30:1.5:1.5:3，v/v/v/v）的展开溶剂系统，在硅胶60 NH2 F254s HPTLC板上进行分离。所提出的HPTLC方法也可能是市场上洋甘菊茶产品质量评估的主要指南。
