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在体外消化模型中对十种可食用植物的总抗氧化能力、多酚含量及淀粉水解酶抑制活性的评估。

Evaluation of the total antioxidant capacity, polyphenol contents and starch hydrolase inhibitory activity of ten edible plants in an in vitro model of digestion.

作者信息

Jayawardena Nilakshi, Watawana Mindani I, Waisundara Viduranga Y

机构信息

Institute of Fundamental Studies, Hantane Road, Kandy, 20000, Sri Lanka.

出版信息

Plant Foods Hum Nutr. 2015 Mar;70(1):71-6. doi: 10.1007/s11130-014-0463-4.

Abstract

The total phenolics contents, total antioxidant capacity (TAC) and starch hydrolase inhibitory activity of the aqueous extracts of 10 edible plants and the stability of these parameters after the gastric and duodenal digestion in an in vitro model was investigated. The TAC was evaluated using the oxygen radical absorbance capacity (ORAC) assay, ferric reducing antioxidant power (FRAP) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH•) and 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) radical scavenging assays. Characterization and quantification of five polyphenol compounds which were previously identified to be present in all the selected plants were carried out. None of the extracts showed a decrease in the total phenolics content or the ORAC and FRAP values following digestion. None of the quantified phenolic compounds had decreased during any of the digestion phases - an observation which was deemed as beneficial in terms of therapeutic properties. Overall, the parameters analyzed were relatively stable throughout the digestive process in all the extracts.

摘要

研究了10种可食用植物水提取物的总酚含量、总抗氧化能力(TAC)和淀粉水解酶抑制活性,以及在体外模型中经胃和十二指肠消化后这些参数的稳定性。使用氧自由基吸收能力(ORAC)测定法、铁还原抗氧化能力(FRAP)、2,2-二苯基-1-苦基肼(DPPH•)和2,2-联氮双(3-乙基苯并噻唑啉-6-磺酸)(ABTS•+)自由基清除测定法评估TAC。对先前鉴定出存在于所有选定植物中的五种多酚化合物进行了表征和定量。消化后,没有一种提取物的总酚含量或ORAC和FRAP值降低。在任何消化阶段,定量的酚类化合物均未减少——就治疗特性而言,这一观察结果被认为是有益的。总体而言,在所有提取物的整个消化过程中,所分析的参数相对稳定。

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