Pierce Campbell Christine M, Kreimer Aimée R, Lin Hui-Yi, Fulp William, O'Keefe Michael T, Ingles Donna J, Abrahamsen Martha, Villa Luisa L, Lazcano-Ponce Eduardo, Giuliano Anna R
Department of Cancer Epidemiology, Moffitt Cancer Center and Research Institute, Tampa, Florida. Center for Infection Research in Cancer, Moffitt Cancer Center and Research Institute, Tampa, Florida.
Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.
Cancer Prev Res (Phila). 2015 Mar;8(3):190-6. doi: 10.1158/1940-6207.CAPR-14-0296. Epub 2015 Jan 9.
Persistent infection with oral HPV16 is believed to drive the development of most oropharyngeal cancers. However, patterns of oral HPV16 persistence remain understudied, particularly among HIV-negative individuals. Oral HPV16 persistence was evaluated among 1,626 participants of the HPV Infection in Men (HIM) Study. Twenty-three oral HPV16-positive men who provided an oral gargle sample on ≥2 study visits were included in the analysis. Archived oral samples from all follow-up visits were tested for HPV16 using Linear Array and INNO-LiPA detection methods. Persistence was evaluated using consecutive HPV16-positive visits held approximately 6 months apart and using the Kaplan-Meier method. Oral HPV16-positive men were aged 18 to 64 years [median, 36 years; interquartile range (IQR), 25-42] and were followed for a median of 44.4 months (IQR, 29.9-49.5). Of 13 incident infections, 4 (30.8%) persisted ≥12 months, 1 (10.0%) persisted ≥24 months, and none persisted ≥36 months [median infection duration, 7.3 months; 95% confidence interval (CI), 6.4-NA)]. Of 10 prevalent infections, 9 (90.0%) persisted ≥12 months, 8 (80.0%) persisted ≥24 months, 4 (57.1%) persisted ≥36 months, and 2 (40.0%) persisted ≥48 months (median infection duration, NA). Twelve-month persistence of incident infections increased significantly with age (Ptrend = 0.028). Prevalent oral HPV16 infections in men persisted longer than newly acquired infections, and persistence appeared to increase with age. These findings may explain the high prevalence of oral HPV observed at older ages. Understanding oral HPV16 persistence will aid in the identification of men at high-risk of developing HPV-related oropharyngeal cancer.
口腔人乳头瘤病毒16型（HPV16）的持续感染被认为是导致大多数口咽癌发生的原因。然而，口腔HPV16持续感染的模式仍未得到充分研究，尤其是在HIV阴性个体中。在男性HPV感染（HIM）研究的1626名参与者中评估了口腔HPV16的持续感染情况。分析纳入了23名在≥2次研究访视时提供了口腔含漱液样本的口腔HPV16阳性男性。使用线性阵列和INNO-LiPA检测方法对所有随访访视的存档口腔样本进行HPV16检测。使用间隔约6个月的连续HPV16阳性访视并采用Kaplan-Meier方法评估持续感染情况。口腔HPV16阳性男性的年龄为18至64岁[中位数为36岁；四分位间距（IQR）为25 - 42]，中位随访时间为44.4个月（IQR为29.9 - 49.5）。在13例新发感染中，4例（30.8%）持续感染≥12个月，1例（10.0%）持续感染≥24个月，无例持续感染≥36个月[中位感染持续时间为7.3个月；95%置信区间（CI）为6.4 - NA]。在10例既往感染中，9例（90.0%）持续感染≥12个月，8例（80.0%）持续感染≥24个月，4例（57.1%）持续感染≥36个月，2例（40.0%）持续感染≥48个月（中位感染持续时间为NA）。新发感染的12个月持续感染率随年龄显著增加（趋势P = 0.028）。男性既往口腔HPV16感染的持续时间长于新获得的感染，且持续感染似乎随年龄增加。这些发现可能解释了在老年人群中观察到的口腔HPV高患病率。了解口腔HPV16的持续感染情况将有助于识别发生HPV相关口咽癌高风险的男性。
