一种用于分析巴西三叶香茶菜三种化学型草药茶的 HPLC 验证方法。

A validated HPLC method for the analysis of herbal teas from three chemotypes of Brazilian Lippia alba.

机构信息

Department of Chemistry, Building of Pharmaceutical Sciences, University of Florence, Via Ugo Schiff, 6, 50019 Sesto Fiorentino (FI), Italy.

出版信息

Food Chem. 2015 May 15;175:366-73. doi: 10.1016/j.foodchem.2014.11.129. Epub 2014 Nov 28.

DOI:10.1016/j.foodchem.2014.11.129

PMID:25577093

Abstract

Infusions and decoctions of three chemotypes of Lippia alba (Mill.) N. E. Brown (Verbenaceae) were investigated for their quantitative profiles by HPLC-DAD-ESI-MS analyses. An RP-HPLC method was developed which permitted the quality control of the preparations. The correct choice of the column allowed the detailed characterization of the constituents in a total analysis time of 35 min. The HPLC method was accordingly validated for linearity range, LOD, LOQ, accuracy and precision. For the quantitative analysis the three major phytochemical groups were taken into consideration, namely iridoids, phenylpropanoids and flavonoids. Comparative quantitative analyses revealed significant differences among the chemotypes that should be taken into account in the uses of the herbal teas. The developed HPLC-UV assay proved to be an efficient and alternative method for the discrimination of the three chemotypes. This is the first report of detailed analysis of the chemical composition of the constituents of L. alba chemotypes' teas.

摘要

采用 HPLC-DAD-ESI-MS 分析方法对三种化学型的白千层（Verbenaceae）的浸剂和煎剂进行定量分析。建立了一种反相高效液相色谱法，可以对制剂进行质量控制。选择合适的柱子可以在总分析时间为 35 分钟的情况下对成分进行详细的特征描述。对 HPLC 方法进行了线性范围、LOD、LOQ、准确度和精密度的验证。对于定量分析，考虑了三个主要的植物化学群，即环烯醚萜类、苯丙素类和黄酮类。比较定量分析显示，化学型之间存在显著差异，在使用草药茶时应考虑这些差异。所开发的 HPLC-UV 测定法被证明是区分三种化学型的有效替代方法。这是首次详细分析白千层化学型茶中成分的化学组成。