Wang Wei, Zhong Zhi-min, Su Ning, Peng Ya-ya, Huang Ting-ting
Department of Clinical Laboratory, Guangzhou First People's Hospital Affiliated to Guangzhou Medical University, China.
Zhonghua Nan Ke Xue. 2014 Nov;20(11):995-8.
To investigate the differences in semen quality between samples collected by masturbation in the clinic and at home.
Based on the WHO guidelines, we analyzed the ejaculates collected by masturbation in the clinic and at home from 342 men under infertility assessment and measured the contents of such biochemical markers in the seminal plasma as neutral α-glucosidase, zinc, and fructose. According to the location of semen collection, we divided the samples into two groups, clinic-collected and home-collected, and analyzed the differences in the semen parameters between the two groups with the SPSS 16.0 software.
Compared with the clinic-collected semen, the home-collected samples had significantly higher mean values in semen volume (4.0 vs 4.9%), sperm concentration (41 vs 64 x 10(6)/ml), total sperm count (175 vs 270 x 10(6) per ejaculate), progressive sperm motility (40 vs 52%), total count of progressively motile sperm (82 vs 135 x 10(6) per ejaculate) (all P <0.05). No significant differences were found between the two groups in normal sperm morphology (4.0 vs 5.0%) and the contents of neutral α-glucosidase (26 vs 24 mU per ejaculate), zinc (8.0 vs 8.0 μmol per ejaculate), and fructose (62 vs 60 μmol per ejaculate) (all P >0.05). Abnormal sperm concentration (<20 x 10(6)/ml) was observed in significantly fewer of the home-collected samples than the clinic-collected ones (18% [62/342] vs 30% [103/342], P<0.05), and so was abnormal progressive sperm motility (<32%) (64% [219/342] vs 75% [256/342], P<0.05).
Our findings show that semen samples collected by masturbation at home has a higher quality than those collected in the clinic. So the location of semen collection should be taken into consideration in infertility investigation.
探讨在诊所手淫采集的样本与在家手淫采集的样本之间精液质量的差异。
根据世界卫生组织指南，我们分析了342名接受不孕症评估的男性在诊所和在家手淫采集的射精样本，并测量了精浆中中性α-葡萄糖苷酶、锌和果糖等生化标志物的含量。根据精液采集地点，我们将样本分为两组，即诊所采集组和家庭采集组，并使用SPSS 16.0软件分析两组精液参数的差异。
与诊所采集的精液相比，家庭采集的样本在精液量（4.0 vs 4.9%）、精子浓度（41 vs 64×10⁶/ml）、总精子数（每次射精175 vs 270×10⁶）、精子前向运动率（40 vs 52%）、前向运动精子总数（每次射精82 vs 135×10⁶）方面的平均值显著更高（所有P<0.05）。两组在正常精子形态（4.0 vs 5.0%）以及中性α-葡萄糖苷酶含量（每次射精26 vs 24 mU）、锌含量（每次射精8.0 vs 8.0 μmol）和果糖含量（每次射精62 vs 60 μmol）方面未发现显著差异（所有P>0.05）。家庭采集样本中精子浓度异常（<20×10⁶/ml）的比例显著低于诊所采集的样本（18%[62/342] vs 30%[103/342]，P<0.05），精子前向运动异常（<32%）的比例也是如此（64%[219/342] vs 75%[256/342]，P<0.05）。
我们的研究结果表明，在家手淫采集的精液样本质量高于在诊所采集的样本。因此，在不孕症调查中应考虑精液采集地点。
