Masoudian Neda, Riazi Gholam Hossein, Afrasiabi Ali, Modaresi Seyed Mohamad Sadegh, Dadras Ali, Rafiei Shahrbanoo, Yazdankhah Meysam, Lyaghi Atiye, Jarah Mostafa, Ahmadian Shahin, Seidkhani Hossein
Institute of Biochemistry and Biophysics (I.B.B.), University of Tehran, Tehran, Iran.
Neurochem Res. 2015 Apr;40(4):629-42. doi: 10.1007/s11064-014-1509-6. Epub 2015 Jan 13.
Glutamate is an excitatory neurotransmitter that is released by the majority of central nervous system synapses and is involved in developmental processes, cognitive functions, learning and memory. Excessive elevated concentrations of Glu in synaptic cleft results in neural cell apoptosis which is called excitotoxicity causing neurodegenerative diseases. Hence, we investigated the possibility of extremely low frequency electromagnetic fields (ELF-EMF) as a risk factor which is able to change Glu concentration in synaptic clef. Synaptosomes as a model of nervous terminal were exposed to ELF-EMF for 15-55 min in flux intensity range from 0.1 to 2 mT and frequency range from 50 to 230 Hz. Finally, all raw data by INForm v4.02 software as an artificial neural network program was analyzed to predict the effect of whole mentioned range spectra. The results showed the tolerance of all effects between the ranges from -35 to +40 % compared to normal state when glutamatergic systems exposed to ELF-EMF. It indicates that glutamatergic system attempts to compensate environmental changes though release or reuptake in order to keep the system safe. Regarding to the wide range of ELF-EMF acquired in this study, the obtained outcomes have potential for developing treatments based on ELF-EMF for some neurological diseases; however, in vivo experiments on the cross linking responses between glutamatergic and cholinergic systems in the presence of ELF-EMF would be needed.
谷氨酸是一种兴奋性神经递质，由大多数中枢神经系统突触释放，参与发育过程、认知功能、学习和记忆。突触间隙中谷氨酸浓度过度升高会导致神经细胞凋亡，这被称为兴奋性毒性，可引发神经退行性疾病。因此，我们研究了极低频电磁场（ELF-EMF）作为一种风险因素改变突触间隙中谷氨酸浓度的可能性。将作为神经末梢模型的突触体暴露于通量强度范围为0.1至2 mT、频率范围为50至230 Hz的ELF-EMF中15至55分钟。最后，使用INForm v4.02软件作为人工神经网络程序对所有原始数据进行分析，以预测整个上述范围频谱的影响。结果表明，当谷氨酸能系统暴露于ELF-EMF时，与正常状态相比，所有效应在-35%至+40%的范围内具有耐受性。这表明谷氨酸能系统试图通过释放或再摄取来补偿环境变化，以保持系统安全。鉴于本研究中获得的ELF-EMF范围广泛，所获得的结果有可能开发基于ELF-EMF的某些神经疾病治疗方法；然而，需要在存在ELF-EMF的情况下对谷氨酸能和胆碱能系统之间的交联反应进行体内实验。
