Zhang Lei, Han Xiao, Cheng Xiang, Tan Xue-Feng, Zhao He-Yan, Zhang Xin-Hua
Department of Human Anatomy, Institute of Neurobiology, Jiangsu Key Laboratory of Neuroregeneration, Medical School, Nantong University, Nantong, Jiangsu Province, China.
Neural Regen Res. 2016 Apr;11(4):597-603. doi: 10.4103/1673-5374.180744.
Fimbria-fornix transection induces both exogenous and endogenous neural stem cells to differentiate into neurons in the hippocampus. This indicates that the denervated hippocampus provides an environment for neuronal differentiation of neural stem cells. However, the pathways and mechanisms in this process are still unclear. Seven days after fimbria fornix transection, our reverse transcription polymerase chain reaction, western blot assay, and enzyme linked immunosorbent assay results show a significant increase in ciliary neurotrophic factor mRNA and protein expression in the denervated hippocampus. Moreover, neural stem cells derived from hippocampi of fetal (embryonic day 17) Sprague-Dawley rats were treated with ciliary neurotrophic factor for 7 days, with an increased number of microtubule associated protein-2-positive cells and decreased number of glial fibrillary acidic protein-positive cells detected. Our results show that ciliary neurotrophic factor expression is up-regulated in the denervated hippocampus, which may promote neuronal differentiation of neural stem cells in the denervated hippocampus.
穹窿海马伞横断术可诱导外源性和内源性神经干细胞在海马中分化为神经元。这表明去神经支配的海马为神经干细胞的神经元分化提供了一个环境。然而，这一过程中的途径和机制仍不清楚。在穹窿海马伞横断术后7天，我们的逆转录聚合酶链反应、蛋白质免疫印迹分析和酶联免疫吸附测定结果显示，去神经支配的海马中睫状神经营养因子的mRNA和蛋白表达显著增加。此外，用来自胎龄17天的Sprague-Dawley大鼠海马的神经干细胞，用睫状神经营养因子处理7天，检测到微管相关蛋白-2阳性细胞数量增加，胶质纤维酸性蛋白阳性细胞数量减少。我们的结果表明，去神经支配的海马中睫状神经营养因子表达上调，这可能促进去神经支配的海马中神经干细胞的神经元分化。
